{"id":2155,"date":"2018-04-20T17:10:09","date_gmt":"2018-04-20T10:10:09","guid":{"rendered":"http:\/\/prc.org.vn\/?p=2155"},"modified":"2018-12-11T17:13:30","modified_gmt":"2018-12-11T10:13:30","slug":"nghien-cuu-xay-dung-quy-trinh-nhan-giong-in-vitro-cay-sam-nui-danh","status":"publish","type":"post","link":"https:\/\/prc.org.vn\/?p=2155","title":{"rendered":"Nghi\u00ean c\u1ee9u x\u00e2y d\u1ef1ng quy tr\u00ecnh nh\u00e2n gi\u1ed1ng in vitro c\u00e2y s\u00e2m N\u00fai D\u00e0nh"},"content":{"rendered":"<p style=\"text-align: justify;\"><span class=\"fontstyle0\">\u00a0 \u00a0 \u00a0 \u00a0Th\u00ed nghi\u1ec7m nghi\u00ean c\u1ee9u x\u00e2y d\u1ef1ng quy tr\u00ecnh nh\u00e2n gi\u1ed1ng c\u00e2y S\u00e2m N\u00fai D\u00e0nh b\u1eb1ng ph\u01b0\u01a1ng ph\u00e1p nu\u00f4i c\u1ea5y m\u00f4 t\u1ebf b\u00e0o nh\u1eb1m cung c\u1ea5p c\u00e2y gi\u1ed1ng ch\u1ea5t l\u01b0\u1ee3ng, s\u1ea1ch b\u1ec7nh v\u00e0 \u1ed5n \u0111\u1ecbnh di truy\u1ec1n g\u00f3p ph\u1ea7n b\u1ea3o t\u1ed3n v\u00e0 ph\u00e1t tri\u1ec3n lo\u00e0i S\u00e2m qu\u00fd n\u00e0y. K\u1ebft qu\u1ea3 nghi\u00ean c\u1ee9u cho th\u1ea5y kh\u1eed tr\u00f9ng m\u1eabu b\u1eb1ng HgCl<\/span><span class=\"fontstyle0\">2 <\/span><span class=\"fontstyle0\">0,1% trong th\u1eddi gian 5 ph\u00fat cho t\u1ef7 l\u1ec7 m\u1eabu s\u1ea1ch b\u1ec7nh \u0111\u1ea1t 95%. M\u1eabu s\u1ea1ch \u0111\u01b0\u1ee3c nu\u00f4i c\u1ea5y tr\u00ean m\u00f4i tr\u01b0\u1eddng t\u00e1i sinh MS b\u1ed5 sung 0,2 mg\/l Ki; 1 mg\/l BA; 100 ml\/l n\u01b0\u1edbc d\u1eeba, 30 g\/l \u0111\u01b0\u1eddng sucroza v\u00e0 6,5 g\/l aga cho t\u1ef7 l\u1ec7 t\u00e1i sinh ch\u1ed3i \u0111\u1ea1t 100%. M\u00f4i tr\u01b0\u1eddng nh\u00e2n nhanh MS b\u1ed5 sung 3 mg\/l BA, 0,2 g\/l IBA, 30 g\/l \u0111\u01b0\u1eddng sucroza v\u00e0 6,5 g\/l aga cho h\u1ec7 s\u1ed1 nh\u00e2n \u0111\u1ea1t 6,6 l\u00e2n. S\u1eed d\u1ee5ng m\u00f4i tr\u01b0\u1eddng ra r\u1ec5 1\/2MS b\u1ed5 sung 1 mg\/l IBA v\u00e0 0,4 mg\/l than ho\u1ea1t t\u00ednh cho t\u1ef7 l\u1ec7 ra r\u1ec5 \u0111\u1ea1t 95%, r\u1ec5 to, m\u1eadp v\u00e0 kh\u1ecfe. C\u00e2y <\/span><span class=\"fontstyle2\">in vitro <\/span><span class=\"fontstyle0\">ho\u00e0n ch\u1ec9nh \u0111\u01b0\u1ee3c \u0111\u01b0a ra v\u01b0\u1eddn \u01b0\u01a1m tr\u00ean n\u1ec1n gi\u00e1 th\u1ec3 h\u1eefu c\u01a1 g\u1ed3m: 50% b\u1ed9t x\u01a1 d\u1eeba v\u00e0 50% ph\u1ebf li\u1ec7u s\u1ea3n xu\u1ea5t n\u1ea5m \u0103n l\u00e0 ph\u00f9 h\u1ee3p nh\u1ea5t v\u1edbi S\u00e2m Nam N\u00fai D\u00e0nh, t\u1ef7 l\u1ec7 s\u1ed1ng \u0111\u1ea1t 94%.<br \/>\n<\/span><strong><span class=\"fontstyle3\">\u00a0 \u00a0 \u00a0 \u00a0T\u1eeb kh\u00f3a<\/span><\/strong><span class=\"fontstyle0\"><strong>:<\/strong> S\u00e2m N\u00fai D\u00e0nh, nu\u00f4i c\u1ea5y m\u00f4 t\u1ebf b\u00e0o, ra r\u1ec5 <\/span><span class=\"fontstyle2\">in vitro<\/span><span class=\"fontstyle0\">, NAA, IBA, BA<\/span><\/p>\n<p style=\"text-align: justify;\">Xem chi ti\u1ebft :\u00a0<a href=\"https:\/\/prc.org.vn\/wp-content\/uploads\/2018\/12\/Sam.nui_.danh_.pdf\">t\u1ea1i \u0111\u00e2y<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u00a0 \u00a0 \u00a0 \u00a0Th\u00ed nghi\u1ec7m nghi\u00ean c\u1ee9u x\u00e2y d\u1ef1ng quy tr\u00ecnh nh\u00e2n gi\u1ed1ng c\u00e2y S\u00e2m N\u00fai D\u00e0nh b\u1eb1ng ph\u01b0\u01a1ng ph\u00e1p<\/p>\n","protected":false},"author":4,"featured_media":2148,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[47],"tags":[],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/prc.org.vn\/index.php?rest_route=\/wp\/v2\/posts\/2155"}],"collection":[{"href":"https:\/\/prc.org.vn\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/prc.org.vn\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/prc.org.vn\/index.php?rest_route=\/wp\/v2\/users\/4"}],"replies":[{"embeddable":true,"href":"https:\/\/prc.org.vn\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=2155"}],"version-history":[{"count":1,"href":"https:\/\/prc.org.vn\/index.php?rest_route=\/wp\/v2\/posts\/2155\/revisions"}],"predecessor-version":[{"id":2156,"href":"https:\/\/prc.org.vn\/index.php?rest_route=\/wp\/v2\/posts\/2155\/revisions\/2156"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/prc.org.vn\/index.php?rest_route=\/wp\/v2\/media\/2148"}],"wp:attachment":[{"href":"https:\/\/prc.org.vn\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=2155"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/prc.org.vn\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=2155"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/prc.org.vn\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=2155"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}