{"id":543,"date":"2017-03-09T10:17:30","date_gmt":"2017-03-09T03:17:30","guid":{"rendered":"http:\/\/demo.prc.maiatech.com.vn\/?p=543"},"modified":"2017-03-09T10:17:31","modified_gmt":"2017-03-09T03:17:31","slug":"nghien-cuu-tham-do-quy-trinh-vi-nhan-giong-hai-loai-hoa-rieng-tia-va-nghe-do-lam-hoa-cay-canh","status":"publish","type":"post","link":"https:\/\/prc.org.vn\/?p=543","title":{"rendered":"Nghi\u00ean c\u1ee9u th\u0103m d\u00f2 quy tr\u00ecnh vi nh\u00e2n gi\u1ed1ng hai lo\u00e0i hoa ri\u1ec1ng t\u00eda v\u00e0 ngh\u1ec7 \u0111\u1ecf l\u00e0m hoa c\u00e2y c\u1ea3nh"},"content":{"rendered":"\n\n\n
\n
1. \u0110\u1eb7t v\u1ea5n \u0111\u1ec1<\/strong><\/span><\/div>\n
\u00a0\u00a0\u00a0\u00a0 Hoa t\u01b0\u01a1i \u0111\u00f3ng m\u1ed9t vai tr\u00f2 quan tr\u1ecdng trong \u0111\u1eddi s\u1ed1ng tinh th\u1ea7n c\u1ee7a con ng\u01b0\u1eddi. X\u00e3 h\u1ed9i c\u00e0ng ph\u00e1t tri\u1ec3n th\u00ec \u0111\u00f2i h\u1ecfi nhu c\u1ea7u v\u1ec1 s\u1ef1 phong ph\u00fa \u0111a d\u1ea1ng c\u1ee7a c\u00e1c lo\u1ea1i hoa ng\u00e0y c\u00e0ng nhi\u1ec1u. Vi\u1ec7t Nam c\u00f3 ngu\u1ed3n t\u00e0i nguy\u00ean di truy\u1ec1n c\u00e2y nhi\u1ec7t \u0111\u1edbi v\u00e0 b\u00e1n nhi\u1ec7t \u0111\u1edbi phong ph\u00fa (Nguy\u1ec5n Ng\u1ecdc Hu\u1ec7, Nguy\u1ec5n Quang Minh 2000), trong \u0111\u00f3 c\u00f3 nhi\u1ec1u gi\u1ed1ng hoa h\u1ecd G\u1eebng (Gingiberaceae<\/em>) mang gi\u00e1 tr\u1ecb th\u1ea9m m\u1ef9 \u0111\u1eb9p. \u0110\u1ec3 ph\u00e1t tri\u1ec3n m\u1ed9t s\u1ed1 gi\u1ed1ng hoa h\u1ecd G\u1eebng theo h\u01b0\u1edbng c\u00e2y c\u1ea3nh th\u00ec c\u00f3 nhi\u1ec1u v\u1ea5n \u0111\u1ec1 c\u1ea7n gi\u1ea3i quy\u1ebft, tr\u01b0\u1edbc h\u1ebft l\u00e0 vi\u1ec7c nghi\u00ean c\u1ee9u nh\u00e2n nhanh t\u1ea1o ra m\u1ed9t s\u1ed1 l\u01b0\u1ee3ng gi\u1ed1ng l\u1edbn. Nh\u1eefng k\u1ebft qu\u1ea3 nghi\u00ean c\u1ee9u v\u1ec1 c\u00e1c lo\u00e0i c\u00e2y h\u1ecd G\u1eebng l\u00e0m hoa ch\u01b0a nhi\u1ec1u (Nguy\u1ec5n Ng\u1ecdc Hu\u1ec7 v\u00e0 Nguy\u1ec5n Quang Minh 1999). Vi\u1ec7c nghi\u00ean c\u1ee9u quy tr\u00ecnh vi nh\u00e2n nhanh gi\u1ed1ng hoa h\u1ecd G\u1eebng l\u00e0 c\u1ea7n thi\u1ebft. Vi\u1ec7c nghi\u00ean c\u1ee9u n\u00e0y kh\u00f4ng ch\u1ec9 c\u00f3 m\u1ee5c \u0111\u00edch t\u1ea1o ra s\u1ed1 l\u01b0\u1ee3ng gi\u1ed1ng l\u1edbn c\u00f3 \u0111\u1ed9 thu\u1ea7n v\u00e0 \u0111\u1ed9 s\u1ea1ch cao m\u00e0 c\u00f2n c\u00f3 \u00fd ngh\u0129a trong vi\u1ec7c b\u1ea3o t\u1ed3n ngu\u1ed3n gen b\u1ea3n \u0111\u1ecba n\u00f3i ri\u00eang v\u00e0 b\u1ea3o t\u1ed3n t\u00e0i nguy\u00ean di truy\u1ec1n th\u1ef1c v\u1eadt n\u00f3i chung.<\/span><\/div>\n
2. N\u1ed9i dung V\u1eadt li\u1ec7u v\u00e0 ph\u01b0\u01a1ng ph\u00e1p nghi\u00ean c\u1ee9u<\/strong><\/span><\/div>\n
2.1 N\u1ed9i dung nghi\u00ean c\u1ee9u<\/span><\/strong><\/em><\/div>\n
\u00a0\u00a0\u00a0 \u00a0 – \u0110\u1ed1i v\u1edbi nghi\u00ean c\u1ee9u t\u1ea1o v\u1eadt li\u1ec7u cho nh\u00e2n nhanh<\/em><\/span><\/div>\n
\u00a0\u00a0\u00a0 \u00a0 Th\u00ed nghi\u1ec7m v\u1ec1 t\u00e1c \u0111\u1ed9ng c\u1ee7a th\u1eddi gian kh\u1eed tr\u00f9ng \u0111\u1ebfn t\u1ef7 l\u1ec7 s\u1ed1ng c\u1ee7a m\u1eabu gi\u1ed1ng<\/span><\/div>\n
\u00a0\u00a0\u00a0\u00a0\u00a0 – \u0110\u1ed1i v\u1edbi nghi\u00ean c\u1ee9u \u1edf giai \u0111o\u1ea1n nh\u00e2n ch\u1ed3i<\/em><\/span><\/div>\n
\u00a0\u00a0\u00a0\u00a0\u00a0 + Th\u00ed nghi\u1ec7m v\u1ec1 t\u00e1c \u0111\u1ed9ng c\u1ee7a BAP \u0111\u1ebfn qu\u00e1 tr\u00ecnh t\u1ea1o ch\u1ed3i.<\/span><\/div>\n
\u00a0\u00a0\u00a0\u00a0 + Th\u00ed nghi\u1ec7m v\u1ec1 t\u00e1c \u0111\u1ed9ng c\u1ee7a t\u1ed5 h\u1ee3p n\u1ed3ng \u0111\u1ed9 t\u1ed1i \u01b0u c\u1ee7a BAP v\u00e0 c\u00e1c n\u1ed3ng \u0111\u1ed9 kh\u00e1c nhau c\u1ee7a IAA \u0111\u1ebfn qu\u00e1 tr\u00ecnh t\u1ea1o ch\u1ed3i.<\/span><\/div>\n
\u00a0\u00a0\u00a0\u00a0\u00a0 – \u0110\u1ed1i v\u1edbi nghi\u00ean c\u1ee9u v\u1ec1 t\u00e1i sinh c\u00e2y ho\u00e0n ch\u1ec9nh.<\/em><\/span><\/div>\n
\u00a0\u00a0\u00a0\u00a0\u00a0 + Th\u00ed nghi\u1ec7m v\u1ec1 t\u00e1c \u0111\u1ed9ng c\u1ee7a NAA \u0111\u1ebfn qu\u00e1 tr\u00ecnh h\u00ecnh th\u00e0nh r\u1ec5.<\/span><\/div>\n
\u00a0\u00a0\u00a0\u00a0\u00a0 – \u0110\u1ed1i v\u1edbi nghi\u00ean c\u1ee9u \u1edf giai \u0111o\u1ea1n \u0111\u01b0a c\u00e2y ra v\u01b0\u1eddn \u01b0\u01a1m.<\/em><\/span><\/div>\n
\u00a0\u00a0\u00a0\u00a0\u00a0 + Th\u00ed nghi\u1ec7m v\u1ec1 s\u1ee9c s\u1ed1ng c\u1ee7a c\u00e2y \u0111\u01b0a t\u1eeb ph\u00f2ng th\u00ed nghi\u1ec7m ra v\u01b0\u1eddn \u01b0\u01a1m.<\/span><\/div>\n
2.2. V\u1eadt li\u1ec7u<\/span><\/strong><\/em><\/div>\n
\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 Hai gi\u1ed1ng c\u1ee7a hai lo\u00e0i hoa h\u1ecd G\u1eebng l\u00e0 hoa Ri\u1ec1ng t\u00eda (Alpinia purpurata<\/em>), k\u00fd hi\u1ec7u VNZ 151 v\u00e0 hoa Ngh\u1ec7 \u0111\u1ecf (Curcuma domestica<\/em>), k\u00fd hi\u1ec7u VNZ 56 trong t\u1eadp \u0111o\u00e0n qu\u1ef9 gen c\u00e2y h\u1ecd G\u1eebng c\u1ee7a Trung t\u00e2m T\u00e0i nguy\u00ean di truy\u1ec1n th\u1ef1c v\u1eadt, Vi\u1ec7n Khoa h\u1ecdc k\u1ef9 thu\u1eadt n\u00f4ng nghi\u1ec7p Vi\u1ec7t Nam.<\/span><\/div>\n
2.3. Ph\u01b0\u01a1ng ph\u00e1p nghi\u00ean c\u1ee9u<\/span><\/strong><\/em><\/div>\n
\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 – C\u00e1c th\u00ed nghi\u1ec7m vi nh\u00e2n gi\u1ed1ng \u0111\u01b0\u1ee3c ti\u1ebfn h\u00e0nh t\u1ea1i Ph\u00f2ng th\u00ed nghi\u1ec7m \u0110a d\u1ea1ng sinh h\u1ecdc c\u1ee7a Trung t\u00e2m T\u00e0i nguy\u00ean di truy\u1ec1n th\u1ef1c v\u1eadt.<\/span><\/div>\n
\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 – Ph\u01b0\u01a1ng ph\u00e1p kh\u1eed tr\u00f9ng: M\u1eabu c\u1ea5y ban \u0111\u1ea7u l\u00e0 ch\u1ed3i c\u00e2y \u0111\u01b0\u1ee3c t\u00e1ch ra t\u1eeb nh\u1eefng c\u00e2y m\u1eb9 kho\u1ebb m\u1ea1nh, s\u1ea1ch b\u1ec7nh. trong t\u1eadp \u0111o\u00e0n c\u00e2y h\u1ecd g\u1eebng. Th\u00ed nghi\u1ec7m kh\u1eed tr\u00f9ng \u1edf c\u00e1c th\u1eddi gian 5, 10, 15, 20, 25 ph\u00fat trong dung d\u1ecbch Clorua thu\u1ef7 ng\u00e2n 0,1% ( HgCl2).<\/span><\/div>\n
\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 – Ph\u01b0\u01a1ng ph\u00e1p nu\u00f4i c\u1ea5y:<\/span><\/div>\n
\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 + C\u01b0\u1eddng \u0111\u1ed9 chi\u1ebfu s\u00e1ng: 2400 lux<\/span><\/div>\n
\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 + Nhi\u1ec7t \u0111\u1ed9 trong ph\u00f2ng nu\u00f4i c\u1ea5y: 20 – 27o<\/sup>C<\/span><\/div>\n
\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 + Quang chu k\u1ef3: 12 gi\u1edd chi\u1ebfu s\u00e1ng -12 gi\u1edd t\u1ed1i<\/span><\/div>\n
\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 – B\u1ed1 tr\u00ed th\u00ed nghi\u1ec7m:<\/span><\/div>\n
\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0+ C\u00e1c th\u00ed nghi\u1ec7m \u0111\u01b0\u1ee3c b\u1ed1 tr\u00ed ng\u1eabu nhi\u00ean, m\u1ed7i c\u00f4ng th\u1ee9c th\u00ed nghi\u1ec7m l\u1eb7p l\u1ea1i 3 l\u1ea7n, m\u1ed7i l\u1ea7n 30 m\u1eabu.<\/span><\/div>\n
\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 + Quan s\u00e1t, theo d\u00f5i th\u00ed nghi\u1ec7m th\u01b0\u1eddng xuy\u00ean b\u1ea3y ng\u00e0y m\u1ed9t l\u1ea7n<\/span><\/div>\n
\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 + S\u1eed d\u1ee5ng ph\u01b0\u01a1ng ph\u00e1p th\u1ed1ng k\u00ea sinh h\u1ecdc \u0111\u1ec3 x\u1eed l\u00fd s\u1ed1 li\u1ec7u th\u00ed nghi\u1ec7m.\u00a0<\/span><\/div>\n
3. K\u1ebft qu\u1ea3 v\u00e0 th\u1ea3o lu\u1eadn<\/strong><\/span><\/div>\n
3.1. T\u1ea1o v\u1eadt li\u1ec7u cho nh\u00e2n nhanh<\/span><\/div>\n
\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 \u0110\u00e2y l\u00e0 giai \u0111o\u1ea1n \u0111\u01b0a m\u1eabu t\u1eeb m\u00f4i tr\u01b0\u1eddng t\u1ef1 nhi\u00ean v\u00e0o trong \u0111i\u1ec1u ki\u1ec7n nu\u00f4i c\u1ea5y in vitro. Giai \u0111o\u1ea1n n\u00e0y \u0111\u01b0\u1ee3c coi l\u00e0 kh\u00e2u k\u1ef9 thu\u1eadt quan tr\u1ecdng t\u1ea1o ti\u1ec1n \u0111\u1ec1 cho q\u00faa tr\u00ecnh nu\u00f4i c\u1ea5y in vitro. Trong giai \u0111o\u1ea1n n\u00e0y c\u1ea7n \u0111\u1ea3m b\u1ea3o y\u00eau c\u1ea7u: t\u1ef7 l\u1ec7 m\u1eabu nhi\u1ec5m th\u1ea5p, t\u1ef7 l\u1ec7 m\u1eabu s\u1ed1ng cao, m\u00f4 t\u1ed3n t\u1ea1i, ph\u00e2n ho\u00e1 v\u00e0 sinh tr\u01b0\u1edfng t\u1ed1t ( Nguy\u1ec5n Quang Th\u1ea1ch, Nguy\u1ec5n L\u00fd Anh 1996 ).<\/span><\/div>\n
\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 K\u1ebft qu\u1ea3 nghi\u00ean c\u1ee9u ph\u01b0\u01a1ng ph\u00e1p kh\u1eed tr\u00f9ng ch\u1ed3i c\u00e2y trong c\u00e1c kho\u1ea3ng th\u1eddi gian kh\u00e1c nhau c\u1ee7a gi\u1ed1ng VNZ 56 v\u00e0 gi\u1ed1ng VNZ 151 b\u1eb1ng Clorua thu\u1ef7 ng\u00e2n 0,1% \u0111\u01b0\u1ee3c tr\u00ecnh b\u00e0y trong B\u1ea3ng 1 (sau b\u1ed1n tu\u1ea7n theo d\u00f5i).<\/span><\/div>\n
B\u1ea3ng 1. \u1ea3nh h\u01b0\u1edfng c\u1ee7a th\u1eddi gian kh\u1eed tr\u00f9ng \u0111\u1ebfn t\u1ef7 l\u1ec7 s\u1ed1ng c\u1ee7a m\u1eabu<\/strong><\/span><\/div>\n
\u00a0<\/strong><\/span><\/p>\n\n\n\n\n\n\n\n\n\n
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TT<\/span><\/div>\n<\/td>\n
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Th\u1eddi gian (ph\u00fat)<\/span><\/div>\n<\/td>\n
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T\u1ef7 l\u1ec7 nhi\u1ec5m (%)<\/span><\/div>\n<\/td>\n
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T\u1ef7 l\u1ec7 ch\u1ebft (%)<\/span><\/div>\n<\/td>\n
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T\u1ef7 l\u1ec7 s\u1ed1ng (%)<\/span><\/div>\n<\/td>\n<\/tr>\n
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Gi\u1ed1ng VNZ56<\/span><\/div>\n<\/td>\n
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Gi\u1ed1ng VNZ151<\/span><\/div>\n<\/td>\n
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Gi\u1ed1ng VNZ56<\/span><\/div>\n<\/td>\n
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Gi\u1ed1ng VNZ151<\/span><\/div>\n<\/td>\n
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Gi\u1ed1ng VNZ56<\/span><\/div>\n<\/td>\n
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Gi\u1ed1ng VNZ151<\/span><\/div>\n<\/td>\n<\/tr>\n
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1<\/span><\/div>\n<\/td>\n
\n
5<\/span><\/div>\n<\/td>\n
\n
100<\/span><\/div>\n<\/td>\n
\n
100<\/span><\/div>\n<\/td>\n
\n
–<\/span><\/div>\n<\/td>\n
\n
–<\/span><\/div>\n<\/td>\n
\n
–<\/span><\/div>\n<\/td>\n
\n
–<\/span><\/div>\n<\/td>\n<\/tr>\n
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2<\/span><\/div>\n<\/td>\n
\n
10<\/span><\/div>\n<\/td>\n
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43<\/span><\/div>\n<\/td>\n
\n
40<\/span><\/div>\n<\/td>\n
\n
–<\/span><\/div>\n<\/td>\n
\n
–<\/span><\/div>\n<\/td>\n
\n
57<\/span><\/div>\n<\/td>\n
\n
60<\/span><\/div>\n<\/td>\n<\/tr>\n
\n
3<\/span><\/div>\n<\/td>\n
\n
15<\/span><\/div>\n<\/td>\n
\n
3<\/span><\/div>\n<\/td>\n
\n
3<\/span><\/div>\n<\/td>\n
\n
14<\/span><\/div>\n<\/td>\n
\n
14<\/span><\/div>\n<\/td>\n
\n
83<\/span><\/div>\n<\/td>\n
\n
83<\/span><\/div>\n<\/td>\n<\/tr>\n
\n
4<\/span><\/div>\n<\/td>\n
\n
20<\/span><\/div>\n<\/td>\n
\n
3<\/span><\/div>\n<\/td>\n
\n
3<\/span><\/div>\n<\/td>\n
\n
47<\/span><\/div>\n<\/td>\n
\n
44<\/span><\/div>\n<\/td>\n
\n
50<\/span><\/div>\n<\/td>\n
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53<\/span><\/div>\n<\/td>\n<\/tr>\n
\n
5<\/span><\/div>\n<\/td>\n
\n
25<\/span><\/div>\n<\/td>\n
\n
0<\/span><\/div>\n<\/td>\n
\n
0<\/span><\/div>\n<\/td>\n
\n
70<\/span><\/div>\n<\/td>\n
\n
67<\/span><\/div>\n<\/td>\n
\n
30<\/span><\/div>\n<\/td>\n
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33<\/span><\/div>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n
\u00a0\u00a0\u00a0\u00a0\u00a0 Th\u1eddi gian kh\u1eed tr\u00f9ng kh\u00e1c nhau c\u00f3 \u1ea3nh h\u01b0\u1edfng l\u1edbn \u0111\u1ebfn t\u1ef7 l\u1ec7 s\u1ed1ng v\u00e0 t\u1ef7 l\u1ec7 nhi\u1ec5m c\u1ee7a m\u1eabu c\u1ea5y. \u1edf c\u00f9ng m\u1ed9t n\u1ed3ng \u0111\u1ed9 ch\u1ea5t kh\u1eed tr\u00f9ng n\u1ebfu \u0111\u1ec3 m\u1eabu c\u1ea5y trong\u00a0th\u1eddi gian 5 ph\u00fat th\u00ec t\u1ef7 l\u1ec7 nhi\u1ec5m cao 100% \u1edf c\u1ea3 hai gi\u1ed1ng. \u1edf ch\u1ebf \u0111\u1ed9 th\u1eddi gian 25 ph\u00fat th\u00ec t\u1ef7 l\u1ec7 ch\u1ebft \u0111\u1ea1t 70% \u1edf gi\u1ed1ng VNZ 56 v\u00e0 67% \u1edf gi\u1ed1ng VNZ 151. Nh\u01b0 v\u1eady ch\u1ebf \u0111\u1ed9 kh\u1eed tr\u00f9ng t\u1ed1i \u01b0u cho c\u1ea3 2 gi\u1ed1ng l\u00e0 HgCl2<\/sub> 0,1% trong 15 ph\u00fat.<\/span><\/div>\n
3.2. Giai \u0111o\u1ea1n nh\u00e2n ch\u1ed3i<\/span><\/div>\n
\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 \u0110\u00e2y l\u00e0 giai \u0111o\u1ea1n \u0111\u00e1nh gi\u00e1 t\u00ednh \u01b0u vi\u1ec7t hay kh\u00f4ng \u01b0u vi\u1ec7t c\u1ee7a ph\u01b0\u01a1ng ph\u00e1p nh\u00e2n gi\u1ed1ng in vitro, n\u00f3 quy\u1ebft \u0111\u1ecbnh h\u1ec7 s\u1ed1 nh\u00e2n c\u1ee7a qui tr\u00ecnh nh\u00e2n. Theo Vuglteke (1989) m\u00f4i tr\u01b0\u1eddng nu\u00f4i c\u1ea5y c\u00f3 b\u1ed5 sung nh\u00f3m Cytokinin th\u00ec c\u00f3 t\u00e1c d\u1ee5ng k\u00edch th\u00edch ta\u1ecd ch\u1ed3i. Trong nu\u00f4i c\u1ea5y m\u00f4 t\u1ebf b\u00e0o th\u1ef1c v\u1eadt BAP l\u00e0 Cytokinin c\u00f3 ho\u1ea1t t\u00ednh sinh h\u1ecdc cao n\u00ean th\u01b0\u1eddng \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng \u0111\u1ec3 th\u00fac \u0111\u1ea9y s\u1ef1 ph\u00e2n chia v\u00e0 l\u1edbn l\u00ean c\u1ee7a c\u00e1c m\u00f4 nu\u00f4i c\u1ea5y. Do \u0111\u00f3 ch\u00fang t\u00f4i \u0111\u00e3 b\u1ed5 sung BAP v\u00e0o m\u00f4i tr\u01b0\u1eddng MS ( Murashige and Skoog 1962), k\u1ebft qu\u1ea3 th\u00ed nghi\u1ec7m tr\u00ean 2 gi\u1ed1ng \u0111\u01b0\u1ee3c tr\u00ecnh b\u00e0y trong B\u1ea3ng 2 (sau t\u00e1m tu\u1ea7n theo d\u00f5i).<\/span><\/div>\n
Qua k\u1ebft qu\u1ea3 b\u1ea3ng 2 cho th\u1ea5y vi\u1ec7c b\u1ed5 sung ch\u1ea5t \u0111i\u1ec1u ti\u1ebft sinh tr\u01b0\u1edfng t\u1ecf ra c\u00f3 hi\u1ec7u qu\u1ea3 trong vi\u1ec7c t\u0103ng h\u1ec7 s\u1ed1 nh\u00e2n. \u1edf c\u00f4ng th\u1ee9c \u0111\u1ed1i ch\u1ee9ng kh\u00f4ng c\u00f3 b\u1ed5 sung BAP th\u00ec h\u1ec7 s\u1ed1 nh\u00e2n ch\u1ec9 \u0111\u1ea1t 2,22 v\u1edbi gi\u1ed1ng VNZ 56 v\u00e0 2,83 v\u1edbi gi\u1ed1ng VNZ 151. Khi t\u0103ng n\u1ed3ng \u0111\u1ed9 BAP t\u1edbi 5 ppm th\u00ec h\u1ec7 s\u1ed1 nh\u00e2n \u0111\u1ea1t 4,33 v\u1edbi gi\u1ed1ng VNZ 56 v\u00e0 6,56 v\u1edbi gi\u1ed1ng VNZ 151. Tuy nhi\u00ean n\u1ebfu ch\u1ec9 quan t\u00e2m \u0111\u1ebfn h\u1ec7 s\u1ed1 nh\u00e2n th\u00f4i th\u00ec ch\u01b0a \u0111\u1ee7 v\u00ec qua theo d\u00f5i ch\u00fang t\u00f4i th\u1ea5y \u1edf n\u1ed3ng \u0111\u1ed9 BAP cao ( 5 ppm) cho k\u1ebft qu\u1ea3 h\u1ec7 s\u1ed1 nh\u00e2n cao nh\u01b0ng chi\u1ec1u cao ch\u1ed3i tr\u1ed9i trung b\u00ecnh l\u1ea1i gi\u1ea3m so v\u1edbi c\u00f4ng th\u1ee9c b\u1ed5 sung BAP \u1edf n\u1ed3ng \u0111\u1ed9 3 ppm. C\u00e1c c\u00f4ng th\u1ee9c b\u1ed5 sung hai n\u1ed3ng \u0111\u1ed9 n\u00e0y th\u00ec ch\u1ea5t l\u01b0\u1ee3ng ch\u1ed3i k\u00e9m h\u01a1n do qu\u00e1 tr\u00ecnh ph\u00e2n ho\u00e1 t\u1ebf b\u00e0o m\u1ea1nh d\u1eabn \u0111\u1ebfn hi\u1ec7n t\u01b0\u1ee3ng ch\u1ed3i k\u00e9m ph\u1ea9m ch\u1ea5t, \u0111\u00e3 th\u1ea5y xu\u1ea5t hi\u1ec7n t\u00ecnh tt\u1ea1ng b\u1ecb d\u00ednh l\u00e1 gi\u1eefa c\u00e1c ch\u1ed3i nh\u01b0ng r\u1ea5t \u00edt, \u0111\u00f3 l\u00e0 m\u1ed9t trong nh\u1eefng bi\u1ec3u hi\u1ec7n c\u1ee7a hi\u1ec7n t\u01b0\u1ee3ng bi\u1ebfn d\u1ecb. Nh\u01b0 v\u1eady c\u00f3 th\u1ec3 \u0111i d\u1ebfn k\u1ebft lu\u1eadn trong giai \u0111o\u1ea1n nh\u1eadn nhanh c\u00e2y hoa h\u1ecd g\u1eebng ch\u1ec9 n\u00ean b\u1ed5 sung ch\u1ea5t \u0111i\u1ec1u ti\u1ebft sinh tr\u01b0\u1edfng \u1edf n\u1ed3ng \u0111\u1ed9 v\u1eeba ph\u1ea3i, m\u00f4i tr\u01b0\u1eddng MS + 3 ppm BAP l\u00e0 c\u00f4ng th\u1ee9c th\u00edch h\u1ee3p cho qu\u00e1 trr\u00ecnh nh\u00e2n nhanh.<\/span><\/div>\n
B\u1ea3ng 2. \u1ea3nh h\u01b0\u1edfng c\u1ee7a BAP \u0111\u1ebfn qu\u00e1 tr\u00ecnh t\u1ea1o ch\u1ed3i<\/strong><\/span><\/div>\n
\u00a0<\/strong><\/span><\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n
\n
TT<\/span><\/div>\n<\/td>\n
\n
Ch\u1ec9 ti\u00eau<\/span><\/div>\n
C\u00f4ng th\u1ee9c<\/span><\/div>\n<\/td>\n
\n
H\u1ec7 s\u1ed1 nh\u00e2n<\/span><\/div>\n<\/td>\n
\n
Chi\u1ec1u cao
\nch\u1ed3i tr\u1ed9i (cm)<\/span><\/div>\n<\/td>\n
\n
Ch\u1ea5t l\u01b0\u1ee3ng ch\u1ed3i<\/span><\/div>\n<\/td>\n<\/tr>\n
\n
Gi\u1ed1ng VNZ56<\/span><\/div>\n<\/td>\n
\n
Gi\u1ed1ng VNZ151<\/span><\/div>\n<\/td>\n
\n
Gi\u1ed1ng VNZ56<\/span><\/div>\n<\/td>\n
\n
Gi\u1ed1ng VNZ151<\/span><\/div>\n<\/td>\n
\n
Gi\u1ed1ng VNZ56<\/span><\/div>\n<\/td>\n
\n
Gi\u1ed1ng VNZ151<\/span><\/div>\n<\/td>\n<\/tr>\n
\n
1<\/span><\/div>\n<\/td>\n
\n
\u0110C: MS<\/span><\/div>\n<\/td>\n
\n
2,22<\/span><\/div>\n<\/td>\n
\n
2,83<\/span><\/div>\n<\/td>\n
\n
8,78<\/span><\/div>\n<\/td>\n
\n
7,53<\/span><\/div>\n<\/td>\n
\n
+++<\/span><\/div>\n<\/td>\n
\n
+++<\/span><\/div>\n<\/td>\n<\/tr>\n
\n
2<\/span><\/div>\n<\/td>\n
\n
\u0110C +1ppm BAP<\/span><\/div>\n<\/td>\n
\n
3,45<\/span><\/div>\n<\/td>\n
\n
4,72<\/span><\/div>\n<\/td>\n
\n
8,50<\/span><\/div>\n<\/td>\n
\n
7,44<\/span><\/div>\n<\/td>\n
\n
+++<\/span><\/div>\n<\/td>\n
\n
+++<\/span><\/div>\n<\/td>\n<\/tr>\n
\n
3<\/span><\/div>\n<\/td>\n
\n
\u0110C + 2ppm BAP<\/span><\/div>\n<\/td>\n
\n
3,83<\/span><\/div>\n<\/td>\n
\n
5,05<\/span><\/div>\n<\/td>\n
\n
8,42<\/span><\/div>\n<\/td>\n
\n
7,00<\/span><\/div>\n<\/td>\n
\n
++<\/span><\/div>\n<\/td>\n
\n
++<\/span><\/div>\n<\/td>\n<\/tr>\n
\n
4<\/span><\/div>\n<\/td>\n
\n
\u0110C + 3ppm BAP<\/span><\/div>\n<\/td>\n
\n
4,11<\/span><\/div>\n<\/td>\n
\n
6,11<\/span><\/div>\n<\/td>\n
\n
7,80<\/span><\/div>\n<\/td>\n
\n
6,72<\/span><\/div>\n<\/td>\n
\n
++<\/span><\/div>\n<\/td>\n
\n
++<\/span><\/div>\n<\/td>\n<\/tr>\n
\n
5<\/span><\/div>\n<\/td>\n
\n
\u0110C + 4ppm BAP<\/span><\/div>\n<\/td>\n
\n
4,22<\/span><\/div>\n<\/td>\n
\n
6,39<\/span><\/div>\n<\/td>\n
\n
7,71<\/span><\/div>\n<\/td>\n
\n
5,14<\/span><\/div>\n<\/td>\n
\n
+<\/span><\/div>\n<\/td>\n
\n
+<\/span><\/div>\n<\/td>\n<\/tr>\n
\n
6<\/span><\/div>\n<\/td>\n
\n
\u0110C + 5ppm BAP<\/span><\/div>\n<\/td>\n
\n
4,33<\/span><\/div>\n<\/td>\n
\n
6,56<\/span><\/div>\n<\/td>\n
\n
6,90<\/span><\/div>\n<\/td>\n
\n
4,90<\/span><\/div>\n<\/td>\n
\n
+<\/span><\/div>\n<\/td>\n
\n
+<\/span><\/div>\n<\/td>\n<\/tr>\n
\n
Trung b\u00ecnh<\/span><\/div>\n<\/td>\n
\n
3,69<\/span><\/div>\n<\/td>\n
\n
5,27<\/span><\/div>\n<\/td>\n
\n
–<\/span><\/div>\n<\/td>\n
\n
–<\/span><\/div>\n<\/td>\n
\n
–<\/span><\/div>\n<\/td>\n
\n
–<\/span><\/div>\n<\/td>\n<\/tr>\n
\n
CV (%)<\/span><\/div>\n<\/td>\n
\n
21,33<\/span><\/div>\n<\/td>\n
\n
26,70<\/span><\/div>\n<\/td>\n
\n
–<\/span><\/div>\n<\/td>\n
\n
–<\/span><\/div>\n<\/td>\n
\n
–<\/span><\/div>\n<\/td>\n
\n
–<\/span><\/div>\n<\/td>\n<\/tr>\n
\n
LSD 0.05<\/sub><\/span><\/div>\n<\/td>\n
\n
0,28<\/span><\/div>\n<\/td>\n
\n
0,18<\/span><\/div>\n<\/td>\n
\n
–<\/span><\/div>\n<\/td>\n
\n
–<\/span><\/div>\n<\/td>\n
\n
–<\/span><\/div>\n<\/td>\n
\n
–<\/span><\/div>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n
(+)<\/sup>.\u00a0\u00a0 Ch\u1ea5t l\u01b0\u1ee3ng ch\u1ed3i trung b\u00ecnh<\/em><\/span><\/div>\n
(++)<\/sup>.\u00a0Ch\u1ea5t l\u01b0\u1ee3ng ch\u1ed3i t\u1ed1t<\/em><\/span><\/div>\n
(+++)<\/sup>. Ch\u1ea5t l\u01b0\u1ee3ng ch\u1ed3i r\u1ea5t t\u1ed1t<\/em><\/span><\/div>\n
\u00a0\u00a0\u00a0\u00a0\u00a0 B\u00ean c\u1ea1nh vi\u1ec7c nghi\u00ean c\u1ee9u \u1ea3nh h\u01b0\u1edfng ri\u00eang r\u1ebd c\u1ee7a ch\u1ea5t \u0111i\u1ec1u ti\u1ebft sinh tr\u01b0\u1edfng ch\u00fang t\u00f4i c\u00f2n nghi\u00ean c\u1ee9u \u1ea3nh h\u01b0\u1edfng c\u1ee7a t\u1ed5 h\u1ee3p c\u00e1c ch\u1ea5t \u0111i\u1ec1u ti\u1ebft sinh tr\u01b0\u1edfng \u0111\u1ebfn qu\u00e1 tr\u00ecnh nh\u00e2n nhanh. K\u1ebft qu\u1ea3 nghi\u00ean c\u1ee9u \u0111\u01b0\u1ee3c tr\u00ecnh b\u00e0y \u1edf B\u1ea3ng 3 (sau t\u00e1m tu\u1ea7n theo d\u00f5i).<\/span><\/div>\n
\u00a0\u00a0\u00a0\u00a0\u00a0 – C\u0169ng nh\u01b0 v\u1edbi th\u00ed nghi\u1ec7m \u0111\u01b0\u1ee3c tr\u00ecnh b\u00e0y trong b\u1ea3ng 2 gi\u1ed1ng VNZ 151 v\u1eabn cho h\u1ec7 s\u1ed1 nh\u00e2n cao h\u01a1n gi\u1ed1ng VNZ 56, \u0111i\u1ec1u n\u00e0y c\u00f3 th\u1ec3 gi\u1ea3i th\u00edch l\u00e0 do b\u1ea3n ch\u1ea5t di truy\u1ec1n c\u1ee7a gi\u1ed1ng.<\/span><\/div>\n
\u00a0\u00a0\u00a0\u00a0\u00a0 – V\u1edbi s\u1ef1 c\u00f3 m\u1eb7t c\u1ee7a t\u1ed5 h\u1ee3p xytokinin + auxin t\u1ef7 l\u1ec7 m\u1eabu \u0111\u1ea1t h\u1ec7 s\u1ed1 nh\u00e2n cao nh\u1ea5t \u1edf t\u1ed5 h\u1ee3p 3,0 ppm BAP + 0,5 ppm IAA l\u00e0 4,78 v\u1edbi gi\u1ed1ng VNZ 56 v\u00e0 6,33 v\u1edbi gi\u1ed1ng VNZ 151.<\/span><\/div>\n
\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 – \u1ede t\u1ed5 h\u1ee3p n\u1ed3ng \u0111\u1ed9 cao c\u1ee7a ch\u1ea5t IAA th\u00ec l\u1ea1i l\u00e0m gi\u1ea3m h\u1ec7 s\u1ed1 nh\u00e2n \u0111\u1ed3ng th\u1eddi ch\u1ea5t l\u01b0\u1ee3ng ch\u1ed3i c\u0169ng k\u00e9m h\u01a1n.<\/span><\/div>\n
B\u1ea3ng 3. \u1ea3nh h\u01b0\u1edfng c\u1ee7a t\u1ed5 h\u1ee3p n\u1ed3ng \u0111\u1ed9 t\u1ed1i \u01b0u c\u1ee7a BAP v\u00e0 c\u00e1c n\u1ed3ng \u0111\u1ed9 kh\u00e1c nhau c\u1ee7a IAA \u0111\u1ebfn qu\u00e1 tr\u00ecnh t\u1ea1o ch\u1ed3i<\/strong><\/span><\/div>\n\n\n\n\n\n\n\n\n\n\n\n
\n
TT<\/span><\/div>\n<\/td>\n
\n
Ch\u1ec9 ti\u00eau<\/span><\/div>\n
C\u00f4ng th\u1ee9c<\/span><\/div>\n<\/td>\n
\n
H\u1ec7 s\u1ed1 nh\u00e2n<\/span><\/div>\n<\/td>\n
\n
Chi\u1ec1u cao ch\u1ed3i tr\u1ed9i (cm)<\/span><\/div>\n<\/td>\n
\n
Ch\u1ea5t l\u01b0\u1ee3ng ch\u1ed3i<\/span><\/div>\n<\/td>\n<\/tr>\n
\n
Gi\u1ed1ng VNZ56<\/span><\/div>\n<\/td>\n
\n
Gi\u1ed1ng VNZ151<\/span><\/div>\n<\/td>\n
\n
Gi\u1ed1ng VNZ56<\/span><\/div>\n<\/td>\n
\n
Gi\u1ed1ng VNZ151<\/span><\/div>\n<\/td>\n
\n
Gi\u1ed1ng VNZ56<\/span><\/div>\n<\/td>\n
\n
Gi\u1ed1ng VNZ151<\/span><\/div>\n<\/td>\n<\/tr>\n
\n
1<\/span><\/div>\n<\/td>\n
\n
\u0110C: MS + 3ppm BAP<\/span><\/div>\n<\/td>\n
\n
4,11<\/span><\/div>\n<\/td>\n
\n
6,11<\/span><\/div>\n<\/td>\n
\n
7,80<\/span><\/div>\n<\/td>\n
\n
6,82<\/span><\/div>\n<\/td>\n
\n
+++<\/span><\/div>\n<\/td>\n
\n
+++<\/span><\/div>\n<\/td>\n<\/tr>\n
\n
2<\/span><\/div>\n<\/td>\n
\n
\u0110C + 0,3ppm IAA<\/span><\/div>\n<\/td>\n
\n
4,33<\/span><\/div>\n<\/td>\n
\n
6,17<\/span><\/div>\n<\/td>\n
\n
7,91<\/span><\/div>\n<\/td>\n
\n
6,90<\/span><\/div>\n<\/td>\n
\n
++<\/span><\/div>\n<\/td>\n
\n
++<\/span><\/div>\n<\/td>\n<\/tr>\n
\n
3<\/span><\/div>\n<\/td>\n
\n
\u0110C + 0,5ppmIAA<\/span><\/div>\n<\/td>\n
\n
4.78<\/span><\/div>\n<\/td>\n
\n
6,33<\/span><\/div>\n<\/td>\n
\n
8,12<\/span><\/div>\n<\/td>\n
\n
7,11<\/span><\/div>\n<\/td>\n
\n
++<\/span><\/div>\n<\/td>\n
\n
++<\/span><\/div>\n<\/td>\n<\/tr>\n
\n
4<\/span><\/div>\n<\/td>\n
\n
\u0110C + 1,0ppmIAA<\/span><\/div>\n<\/td>\n
\n
4,22<\/span><\/div>\n<\/td>\n
\n
6,17<\/span><\/div>\n<\/td>\n
\n
6,70<\/span><\/div>\n<\/td>\n
\n
5,67<\/span><\/div>\n<\/td>\n
\n
+<\/span><\/div>\n<\/td>\n
\n
+<\/span><\/div>\n<\/td>\n<\/tr>\n
\n
Trung b\u00ecnh<\/span><\/div>\n<\/td>\n
\n
4,36<\/span><\/div>\n<\/td>\n
\n
6,19<\/span><\/div>\n<\/td>\n
\n
–<\/span><\/div>\n<\/td>\n
\n
–<\/span><\/div>\n<\/td>\n
\n
–<\/span><\/div>\n<\/td>\n
\n
–<\/span><\/div>\n<\/td>\n<\/tr>\n
\n
CV (%)<\/span><\/div>\n<\/td>\n
\n
6,74<\/span><\/div>\n<\/td>\n
\n
1,52<\/span><\/div>\n<\/td>\n
\n
–<\/span><\/div>\n<\/td>\n
\n
–<\/span><\/div>\n<\/td>\n
\n
–<\/span><\/div>\n<\/td>\n
\n
–<\/span><\/div>\n<\/td>\n<\/tr>\n
\n
LSD 0.05<\/sub><\/span><\/div>\n<\/td>\n
\n
0,24<\/span><\/div>\n<\/td>\n
\n
0,44<\/span><\/div>\n<\/td>\n
\n
–<\/span><\/div>\n<\/td>\n
\n
–<\/span><\/div>\n<\/td>\n
\n
–<\/span><\/div>\n<\/td>\n
\n
–<\/span><\/div>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n
(+)<\/sup>.\u00a0\u00a0\u00a0 Ch\u1ea5t l\u01b0\u1ee3ng r\u1ec5 trung b\u00ecnh<\/em><\/span><\/div>\n
(++)<\/sup>.\u00a0Ch\u1ea5t l\u01b0\u1ee3ng r\u1ec5 t\u1ed1t<\/em><\/span><\/div>\n
(+++)<\/sup>. Ch\u1ea5t l\u01b0\u1ee3ng r\u1ec5 r\u1ea5t t\u1ed1t<\/em><\/span><\/div>\n
\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0<\/span><\/div>\n
4. Giai \u0111o\u1ea1n t\u1ea1o c\u00e2y ho\u00e0n ch\u1ec9nh<\/span><\/div>\n
\u00a0\u00a0\u00a0 Trong giai \u0111o\u1ea1n n\u00e0y ng\u01b0\u1eddi ta th\u01b0\u1eddng b\u1ed5 sung v\u00e0o m\u00f4i tr\u01b0\u1eddng nu\u00f4i c\u1ea5y c\u00e1c ch\u1ea5t k\u00edch th\u00edch sinh tr\u01b0\u1edfng thu\u1ed9c nh\u00f3m auxin (NAA, 2,4D…) v\u1edbi n\u1ed3ng \u0111\u1ed9 th\u00edch h\u1ee3p \u0111\u1ec3 t\u1ea1o r\u1ec5 cho ch\u1ed3i in vitro t\u1ea1o c\u00e2y ho\u00e0n ch\u1ec9nh \u0111\u1ea1t ti\u00eau chu\u1ea9n c\u00e2y ra v\u01b0\u1eddn \u01b0\u01a1m.<\/span><\/div>\n
B\u1ea3ng 4. \u1ea3nh h\u01b0\u1edfng c\u1ee7a NAA \u0111\u1ebfn qu\u00e1 tr\u00ecnh h\u00ecnh th\u00e0nh r\u1ec5<\/strong><\/span><\/div>\n
\u00a0<\/strong><\/span><\/div>\n\n\n\n\n\n\n\n\n
\n
TT<\/span><\/div>\n<\/td>\n
\n
Ch\u1ec9 t\u00eau<\/span><\/div>\n
C\u00f4ng th\u1ee9c<\/span><\/div>\n<\/td>\n
\n
T\u1ef7 l\u1ec7 ra r\u1ec5 (%)<\/span><\/div>\n<\/td>\n
\n
S\u1ed1 r\u1ec5
\ntrung b\u00ecnh\/c\u00e2y<\/span><\/div>\n<\/td>\n
\n
Ch\u1ea5t l\u01b0\u1ee3ng r\u1ec5<\/span><\/div>\n<\/td>\n<\/tr>\n
\n
Gi\u1ed1ng VNZ56<\/span><\/div>\n<\/td>\n
\n
Gi\u1ed1ng VNZ151<\/span><\/div>\n<\/td>\n
\n
Gi\u1ed1ng VNZ56<\/span><\/div>\n<\/td>\n
\n
Gi\u1ed1ng VNZ151<\/span><\/div>\n<\/td>\n
\n
Gi\u1ed1ng VNZ56<\/span><\/div>\n<\/td>\n
\n
Gi\u1ed1ng VNZ151<\/span><\/div>\n<\/td>\n<\/tr>\n
\n
1<\/span><\/div>\n<\/td>\n
\n
\u0110C: MS<\/span><\/div>\n<\/td>\n
\n
100<\/span><\/div>\n<\/td>\n
\n
100<\/span><\/div>\n<\/td>\n
\n
3,7<\/span><\/div>\n<\/td>\n
\n
3,4<\/span><\/div>\n<\/td>\n
\n
++<\/span><\/div>\n<\/td>\n
\n
++<\/span><\/div>\n<\/td>\n<\/tr>\n
\n
2<\/span><\/div>\n<\/td>\n
\n
\u0110C +0,3ppm NAA<\/span><\/div>\n<\/td>\n
\n
100<\/span><\/div>\n<\/td>\n
\n
100<\/span><\/div>\n<\/td>\n
\n
4,6<\/span><\/div>\n<\/td>\n
\n
4,1<\/span><\/div>\n<\/td>\n
\n
++<\/span><\/div>\n<\/td>\n
\n
++<\/span><\/div>\n<\/td>\n<\/tr>\n
\n
3<\/span><\/div>\n<\/td>\n
\n
\u0110C +0,5ppm NAA<\/span><\/div>\n<\/td>\n
\n
100<\/span><\/div>\n<\/td>\n
\n
100<\/span><\/div>\n<\/td>\n
\n
5,3<\/span><\/div>\n<\/td>\n
\n
4,6<\/span><\/div>\n<\/td>\n
\n
+++<\/span><\/div>\n<\/td>\n
\n
+++<\/span><\/div>\n<\/td>\n<\/tr>\n
\n
4<\/span><\/div>\n<\/td>\n
\n
\u0110C +1,0ppm NAA<\/span><\/div>\n<\/td>\n
\n
100<\/span><\/div>\n<\/td>\n
\n
100<\/span><\/div>\n<\/td>\n
\n
3,9<\/span><\/div>\n<\/td>\n
\n
3,7<\/span><\/div>\n<\/td>\n
\n
+<\/span><\/div>\n<\/td>\n
\n
+<\/span><\/div>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n
(+)<\/sup>.\u00a0\u00a0\u00a0 Ch\u1ea5t l\u01b0\u1ee3ng r\u1ec5 trung b\u00ecnh<\/em><\/span><\/div>\n
(++)<\/sup>.\u00a0Ch\u1ea5t l\u01b0\u1ee3ng r\u1ec5 t\u1ed1t<\/em><\/span><\/div>\n
(+++)<\/sup>. Ch\u1ea5t l\u01b0\u1ee3ng r\u1ec5 r\u1ea5t t\u1ed1t<\/em><\/span><\/div>\n
\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 K\u1ebft qu\u1ea3 sau b\u1ed1n tu\u1ea7n theo d\u00f5i\u00a0\u1edf B\u1ea3ng 4 nh\u01b0 sau:<\/span><\/div>\n
\u00a0\u00a0\u00a0\u00a0 – \u0110\u1ed1i v\u1edbi hai gi\u1ed1ng VNZ 56 v\u00e0 VNZ 151 th\u00ec ch\u1ea5t \u0111i\u1ec1u ti\u1ebft sinh tr\u01b0\u1edfng kh\u00f4ng c\u00f3 \u1ea3nh h\u01b0\u1edfng nhi\u1ec1u \u0111\u1ebfn t\u1ef7 l\u1ec7 ra r\u1ec5 c\u1ee7a ch\u1ed3i in vitro, th\u1ec3 hi\u1ec7n l\u00e0 \u1edf c\u1ea3 4 c\u00f4ng th\u1ee9c \u0111\u1ec1u \u0111\u1ea1t t\u1ef7 l\u1ec7 ra r\u1ec5 l\u00e0 100%.<\/span><\/div>\n
\u00a0\u00a0\u00a0\u00a0 – S\u1ed1 l\u01b0\u1ee3ng r\u1ec5 trung b\u00ecnh tr\u00ean m\u1ed9t c\u00e2y thay \u0111\u1ed5i tu\u1ef3 theo n\u1ed3ng \u0111\u1ed9 c\u1ee7a NAA, \u1edf c\u00f4ng th\u1ee9c \u0111\u1ed1i ch\u1ee9ng kh\u00f4ng b\u1ed5 sung NAA s\u1ed1 r\u1ec5 trung b\u00ecnh l\u00e0 3,7 c\u00f2n \u1edf c\u00f4ng th\u1ee9c b\u1ed5 sung 0,5 ppm NAA th\u00ec s\u1ed1 r\u1ec5 trung b\u00ecnh \u0111\u1ea1t 5,3 v\u1edbi gi\u1ed1ng VNZ 56 v\u00e0 4,6 v\u1edbi gi\u1ed1ng VNZ151. \u1edf n\u1ed3ng \u0111\u1ed9 1,0 ppm NAA \u0111\u00e3 \u1ee9c ch\u1ebf s\u1ef1 ra r\u1ec5 c\u1ee7a ch\u1ed3i in vitro \u0111\u1ed3ng th\u1eddi chi\u1ec1u d\u00e0i r\u1ec5 c\u0169ng gi\u1ea3m \u0111i. Nh\u01b0 v\u1eady \u0111\u1ec3 t\u1ea1o c\u00e2y ho\u00e0n ch\u1ec9nh cho ch\u1ed3i in vitro 2 gi\u1ed1ng hoa h\u1ecd G\u1eebng th\u00ec m\u00f4i tr\u01b0\u1eddng th\u00edch h\u1ee3p l\u00e0 MS + 0,5 ppm NAA.<\/span><\/div>\n
5. Giai \u0111o\u1ea1n \u0111\u01b0a c\u00e2y ra v\u01b0\u1eddn \u01b0\u01a1m<\/span><\/div>\n
\u00a0\u00a0\u00a0\u00a0 \u0110\u00e2y l\u00e0 giai \u0111o\u1ea1n cu\u1ed1i c\u00f9ng c\u1ee7a qui tr\u00ecnh nh\u00e2n gi\u1ed1ng in vitro, n\u00f3 c\u00f3 \u00fd ngh\u0129a v\u00f4 c\u00f9ng quan tr\u1ecdng quy\u1ebft \u0111\u1ecbnh kh\u1ea3 n\u0103ng \u1ee9ng d\u1ee5ng c\u1ee7a to\u00e0n b\u1ed9 qu\u00e1 tr\u00ecnh nu\u00f4i c\u1ea5y in vitro v\u00e0o th\u1ef1c ti\u1ec5n s\u1ea3n xu\u1ea5t (Martinus Nijhoff 1987). Giai \u0111o\u1ea1n n\u00e0y l\u00e0 chuy\u1ec3n c\u00e2y t\u1eeb m\u00f4i tr\u01b0\u1eddng nh\u00e2n t\u1ea1o sang m\u00f4i tr\u01b0\u1eddng t\u1ef1 nhi\u00ean, t\u1ee9c l\u00e0 c\u00e2y ph\u1ea3i chy\u1ec3n t\u1eeb tr\u1ea1ng th\u00e1i d\u1ecb d\u01b0\u1ee1ng sang tr\u1ea1ng th\u00e1i t\u1ef1 d\u01b0\u1ee1ng. Ch\u00ednh v\u00ec v\u1eady vi\u1ec7c t\u00ecm ra gi\u00e1 th\u1ec3 th\u00edch h\u1ee3p \u0111\u1ec3 ra c\u00e2y l\u00e0 c\u1ea7n thi\u1ebft. Ch\u00fang t\u00f4i \u0111\u00e3 nghi\u00ean c\u1ee9u \u1ea3nh h\u01b0\u1edfng c\u1ee7a hai lo\u1ea1i gi\u00e1 th\u1ec3 l\u00e0 \u0111\u1ea5t v\u00e0 tr\u1ea5u hun, k\u1ebft qu\u1ea3 \u0111\u01b0\u1ee3c tr\u00ecnh b\u00e0y trong b\u1ea3ng s\u00e1u.<\/span><\/div>\n
B\u1ea3ng 6. \u1ea3nh h\u01b0\u1edfng c\u1ee7a gi\u00e1 th\u1ec3 \u0111\u1ebfn s\u1ee9c s\u1ed1ng c\u1ee7a c\u00e2y<\/strong><\/span><\/div>\n
\n\n\n\n\n\n\n
\n
TT<\/span><\/div>\n<\/td>\n
\n
C\u00f4ng th\u1ee9c<\/span><\/div>\n<\/td>\n
\n
T\u1ef7 l\u1ec7 s\u1ed1ng (%)<\/span><\/div>\n<\/td>\n<\/tr>\n
\n
Gi\u1ed1ng VNZ 56<\/span><\/div>\n<\/td>\n
\n
Gi\u1ed1ng VNZ151<\/span><\/div>\n<\/td>\n<\/tr>\n
\n
1<\/span><\/div>\n<\/td>\n
\n
Tr\u1ea5u hun<\/span><\/div>\n<\/td>\n
\n
97<\/span><\/div>\n<\/td>\n
\n
100<\/span><\/div>\n<\/td>\n<\/tr>\n
\n
2<\/span><\/div>\n<\/td>\n
\n
\u0110\u1ea5t<\/span><\/div>\n<\/td>\n
\n
83<\/span><\/div>\n<\/td>\n
\n
87<\/span><\/div>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n
\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 Qua b\u1ea3ng 6 ch\u00fang t\u00f4i th\u1ea5y gi\u00e1 th\u1ec3 c\u00f3 \u1ea3nh h\u01b0\u1edfng nhi\u1ec1u \u0111\u1ebfn t\u1ef7 l\u1ec7 s\u1ed1ng c\u1ee7a c\u00e2y in vitro. Gi\u00e1 th\u1ec3 tr\u1ea5u hun t\u1ecf ra c\u00f3 hi\u1ec7u qu\u1ea3 cho t\u1ef7 l\u1ec7 s\u1ed1ng cao 100% v\u1edbi gi\u1ed1ng VNZ 151 v\u00e0 97% v\u1edbi gi\u1ed1ng VNZ 56, b\u1edfi v\u00ec tr\u1ea5u hun l\u00e0 lo\u1ea1i gi\u00e1 th\u1ec3 th\u00f4ng tho\u00e1ng v\u00e0 tho\u00e1t n\u01b0\u1edbc.<\/span><\/div>\n
4. K\u1ebft lu\u1eadn v\u00e0 \u0111\u1ec1 ngh\u1ecb<\/strong><\/span><\/div>\n
1. K\u1ebft lu\u1eadn<\/span><\/div>\n
\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 – C\u00f3 th\u1ec3 nh\u00e2n nhanh 2 gi\u1ed1ng hoa h\u1ecd g\u1eebng b\u1eb1ng ph\u01b0\u01a1ng ph\u00e1p nu\u00f4i c\u1ea5y m\u00f4, t\u1eeb \u0111\u00f3 t\u1ea1o ti\u1ec1n \u0111\u1ec1 cho qu\u00e1 tr\u00ecnh s\u1eed d\u1ee5ng c\u00e1c ngu\u1ed3n gen \u0111ang l\u01b0u gi\u01b0 \u1edf Ng\u00e2n h\u00e0ng gen in vitro.<\/span><\/div>\n
\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 – <\/strong>\u00a0Ch\u1ebf \u0111\u1ed9 kh\u1eed tr\u00f9ng th\u00edch h\u1ee3p nh\u1ea5t cho c\u1ea3 2 gi\u1ed1ng hoa h\u1ecd g\u1eebng VNZ 56 v\u00e0 VNZ 151 l\u00e0 HgCl\u00ad2 0,1% trong th\u1eddi gian 15 ph\u00fat.<\/span><\/div>\n
\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 – <\/strong>\u00a0Ch\u1ea5t \u0111i\u1ec1u ti\u1ebft sinh tr\u01b0\u1edfng c\u00f3 \u1ea3nh h\u01b0\u1edfng nhi\u1ec1u \u0111\u1ebfn h\u1ec7 s\u1ed1 nh\u00e2n c\u1ee7a ch\u1ed3i in vitro tr\u00ean c\u1ea3 2 gi\u1ed1ng VNZ 56 v\u00e0 VNZ 151. H\u1ec7 s\u1ed1 nh\u00e2n \u0111\u1ed1i v\u1edbi gi\u1ed1ng VNZ 56 l\u00e0 4,78, v\u1edbi gi\u1ed1ng VNZ 151 l\u00e0 6,33. M\u00f4i tr\u01b0\u1eddng th\u00edch h\u1ee3p cho giai \u0111o\u1ea1n nh\u00e2n nhanh l\u00e0:<\/span><\/div>\n
MS + 3,0 ppm BAP + 0,5 ppm IAA + 3% \u0111\u01b0\u1eddng + 6,5g agar\/l\u00edt<\/span><\/div>\n
\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 – <\/strong>\u00a0M\u00f4i tr\u01b0\u1eddng ph\u00f9 h\u1ee3p \u0111\u1ec3 t\u1ea1o c\u00e2y ho\u00e0n ch\u1ec9nh v\u1edbi hai gi\u1ed1ng VNZ 56 v\u00e0 VNZ 151 l\u00e0:<\/span><\/div>\n
MS + 0,5 ppm NAA + 3% \u0111\u01b0\u1eddng + 6,5g agar\/l\u00edt<\/span><\/div>\n
\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 – <\/strong>\u00a0Gi\u00e1 th\u1ec3 th\u00edch h\u1ee3p tr\u1ed3ng c\u00e2y in vitro ngo\u00e0i v\u01b0\u1eddn \u01b0\u01a1m l\u00e0 gi\u00e1 th\u1ec3 tr\u1ea5u hun.<\/span><\/div>\n
\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 – <\/strong>Th\u1eddi gian t\u1eeb khi \u0111\u01b0a m\u1ed9t ch\u1ed3i c\u00e2y v\u00e0o nu\u00f4i c\u1ea5y in vitro \u0111\u1ebfn khi \u0111\u01b0a c\u00e2y tr\u1ed3ng ra ngo\u00e0i ru\u1ed9ng l\u00e0 6 th\u00e1ng.<\/span><\/div>\n
2. \u0110\u1ec1 ngh\u1ecb<\/span><\/div>\n
\u00a0\u00a0\u00a0\u00a0\u00a0 – S\u1eed d\u1ee5ng quy tr\u00ecnh vi nh\u00e2n gi\u1ed1ng \u0111\u1ec3 nh\u00e2n hai lo\u00e0i hoa h\u1ecd G\u1eebng l\u00e0 hoa Ri\u1ec1ng t\u00eda v\u00e0 hoa Ngh\u1ec7 \u0111\u1ecf l\u00e0m hoa c\u00e2y c\u1ea3nh.<\/span><\/div>\n
\u00a0\u00a0\u00a0\u00a0\u00a0 – Ti\u1ebfp t\u1ee5c m\u1edf r\u1ed9ng nghi\u00ean c\u1ee9u c\u00e1c lo\u1ea1i gi\u00e1 th\u1ec3 tr\u1ed3ng ngo\u00e0i v\u01b0\u1eddn \u01b0\u01a1m cho hai lo\u00e0i c\u00e2y h\u1ecd G\u1eebng.<\/span><\/div>\n
\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 – Ti\u1ebfp t\u1ee5c nghi\u00ean c\u1ee9u s\u1ef1 sinh tr\u01b0\u1edfng v\u00e0 ph\u00e1t tri\u1ec3n c\u1ee7a hai lo\u00e0i hoa h\u1ecd G\u1eebng khi tr\u1ed3ng ngo\u00e0i \u0111\u1ed3ng ru\u1ed9ng.<\/span><\/div>\n
\u00a0<\/strong><\/span><\/div>\n
S\u01a1 \u0111\u1ed3 Qui tr\u00ecnh vi nh\u00e2n gi\u1ed1ng<\/strong><\/span><\/div>\n
hoa Ri\u1ec1ng t\u00eda v\u00e0 Ngh\u1ec7 \u0111\u1ecf<\/strong><\/span><\/div>\n
\u00a0<\/strong><\/span><\/div>\n
C\u00e2y m\u1eb9 (ch\u1ed3i c\u00e2y)<\/strong><\/span><\/div>\n
\u00a0<\/span><\/div>\n\n\n\n
\n
\u00a0<\/span><\/div>\n<\/td>\n
\n
HgCl2 0,1%, 15ph\u00fat<\/span><\/div>\n
MS + 3% \u0111\u01b0\u1eddng + 6,5g\/l\u00edt agar<\/span><\/div>\n
\u00a0<\/span><\/div>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n
Kh\u1eed tr\u00f9ng\u00a0\u00a0\u00a0\u00a0<\/strong><\/span><\/div>\n\n\n\n
\n

\u00a0\u00a0\u00a0\u00a0<\/span><\/div>\n<\/td>\n
\n
MS + 3ppm BAP + 0,5ppm IAA + 3% \u0111\u01b0\u1eddng + 6,5g\/l\u00edt agar<\/span><\/div>\n
\u00a0<\/span><\/div>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n
\u00a0\u00a0Nh\u00e2n nhanh ch\u1ed3i<\/strong><\/span><\/div>\n\n\n\n
\n

\u00a0\u00a0\u00a0\u00a0<\/span><\/div>\n<\/td>\n
\n
MS + 0,5 ppm NAA + 3% \u0111\u01b0\u1eddng + 6,5g\/l\u00edt agar<\/span><\/div>\n
\u00a0<\/span><\/div>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n
C\u00e2y ho\u00e0n ch\u1ec9nh<\/strong><\/span><\/div>\n\n\n\n\n
\n

\u00a0<\/span><\/div>\n<\/td>\n
\n
Gi\u00e1 th\u1ec3 tr\u1ea5u hun<\/span><\/div>\n
\u00a0<\/span><\/div>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n
V\u01b0\u1eddn \u01b0\u01a1m<\/strong><\/span><\/div>\n
\u00a0<\/span><\/div>\n

Quy tr\u00ecnh vi nh\u00e2n gi\u1ed1ng hoa ri\u1ec1ng t\u00eda v\u00e0 ngh\u1ec7 \u0111\u1ecf<\/span><\/p>\n

1. L\u1ea5y m\u1eabu:<\/strong><\/span><\/div>\n
\u00a0\u00a0\u00a0\u00a0 Ch\u1ecdn nh\u1eefng ch\u1ed3i c\u00e2y c\u00f3 chi\u1ec1u cao kho\u1ea3ng 2 – 3 cm t\u1eeb nh\u1eefng c\u00e2y m\u1eb9 sinh tr\u01b0\u1edfng t\u1ed1t, kh\u00f4ng b\u1ecb s\u00e2u b\u1ec7nh.<\/span><\/div>\n
2. Kh\u1eed tr\u00f9ng:<\/strong><\/span><\/div>\n
\u00a0\u00a0\u00a0 Ch\u1ed3i c\u00e2y \u0111\u01b0\u1ee3c c\u1eaft v\u00e0 \u0111em r\u1eeda s\u1ea1ch, sau \u0111\u00f3 ng\u00e2m trong dung d\u1ecbch HgCl2 0,1%, th\u1eddi gian 15 ph\u00fat trong \u0111i\u1ec1u ki\u1ec7n nu\u00f4i c\u1ea5y v\u00f4 tr\u00f9ng.<\/span><\/div>\n
3. Nh\u00e2n nhanh ch\u1ed3i:<\/strong><\/span><\/div>\n
\u00a0\u00a0\u00a0 Nh\u1eefng ch\u1ed3i \u0111\u00e3 s\u1ed1ng v\u00e0 kh\u00f4ng b\u1ecb nhi\u1ec5m \u0111\u01b0\u1ee3c \u0111\u01b0a v\u00e0o nh\u00e2n nhanh trong m\u00f4i tr\u01b0\u1eddng c\u01a1 b\u1ea3n l\u00e0 MS c\u00f3 b\u1ed5 sung t\u1ed5 h\u1ee3p ch\u1ea5t \u0111i\u1ec1u ti\u1ebft sinh tr\u01b0\u1edfng 3ppm BAP + 0,5ppm IAA. Th\u1eddi gian nh\u00e2n nhanh ch\u1ed3i kho\u1ea3ng 60 ng\u00e0y.<\/span><\/div>\n
4. T\u1ea1o c\u00e2y ho\u00e0n ch\u1ec9nh:<\/strong><\/span><\/div>\n
\u00a0\u00a0\u00a0\u00a0 C\u1ee5m ch\u1ed3i h\u00ecnh th\u00e0nh trong giai \u0111o\u1ea1n nh\u00e2n ch\u1ed3i s\u1ebd \u0111\u01b0\u1ee3c t\u00e1ch ra v\u00e0 c\u1ea5y v\u00e0o m\u00f4i tr\u01b0\u1eddng MS c\u00f3 b\u1ed5 sung 0,5 ppm NAA \u0111\u1ec3 h\u00ecnh th\u00e0nh r\u1ec5 t\u1ea1o c\u00e2y ho\u00e0n ch\u1ec9nh.<\/span><\/div>\n
5. Tr\u1ed3ng c\u00e2y ra v\u01b0\u1eddn \u01b0\u01a1m:<\/strong><\/span><\/div>\n
\u00a0\u00a0\u00a0\u00a0 C\u00e2y c\u00f3 chi\u1ec1u cao kho\u1ea3ng 3 – 5 cm, s\u1ed1 r\u1ec5 kho\u1ea3ng 4 – 5 c\u00e1i \u0111\u01b0\u1ee3c \u0111\u01b0a ra tr\u1ed3ng trong v\u01b0\u1eddn \u01b0\u01a1m tr\u00ean gi\u00e1 th\u1ec3 tr\u1ea5u hun ho\u1eb7c \u0111\u1ea5t ph\u00f9 sa. Sau 1 th\u00e1ng chuy\u1ec3n ra tr\u1ed3ng ngo\u00e0i \u0111\u1ed3ng ru\u1ed9ng.\u00a0<\/span><\/div>\n
\u00a0<\/span><\/div>\n
\n
\u00a0<\/span>C\u00e1c t\u1eeb vi\u1ebft t\u1eaft<\/strong><\/span><\/div>\n<\/div>\n
\u00a0<\/strong><\/span><\/div>\n
BAP<\/strong>:\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 \u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 6 – Benzylaminopurine<\/span><\/div>\n
\u0110C:<\/strong>\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 \u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 \u0110\u1ed1i ch\u1ee9ng<\/span><\/div>\n
IAA<\/strong>:\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 \u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 Indole-3-acetic acid<\/span><\/div>\n
MS:<\/strong>\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 \u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 Murashige and Skoog<\/span><\/div>\n
NAA<\/strong>:\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 \u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 Naphtyl acetic acid
\n<\/span><\/div>\n<\/td>\n<\/tr>\n
PGS, TS L\u01b0u Ng\u1ecdc Tr\u00ecnh, TS Nguy\u1ec5n Th\u1ecb Ng\u1ecdc Hu\u1ec7, ThS Tr\u1ea7n Danh S\u1eedu, KS Ho\u00e0ng Th\u1ecb Hu\u1ec7<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n","protected":false},"excerpt":{"rendered":"

1. \u0110\u1eb7t v\u1ea5n \u0111\u1ec1 \u00a0\u00a0\u00a0\u00a0 Hoa t\u01b0\u01a1i \u0111\u00f3ng m\u1ed9t vai tr\u00f2 quan tr\u1ecdng trong \u0111\u1eddi s\u1ed1ng tinh th\u1ea7n c\u1ee7a con<\/p>\n","protected":false},"author":4,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[47],"tags":[],"_links":{"self":[{"href":"https:\/\/prc.org.vn\/index.php?rest_route=\/wp\/v2\/posts\/543"}],"collection":[{"href":"https:\/\/prc.org.vn\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/prc.org.vn\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/prc.org.vn\/index.php?rest_route=\/wp\/v2\/users\/4"}],"replies":[{"embeddable":true,"href":"https:\/\/prc.org.vn\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=543"}],"version-history":[{"count":1,"href":"https:\/\/prc.org.vn\/index.php?rest_route=\/wp\/v2\/posts\/543\/revisions"}],"predecessor-version":[{"id":544,"href":"https:\/\/prc.org.vn\/index.php?rest_route=\/wp\/v2\/posts\/543\/revisions\/544"}],"wp:attachment":[{"href":"https:\/\/prc.org.vn\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=543"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/prc.org.vn\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=543"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/prc.org.vn\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=543"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}