{"id":562,"date":"2017-03-09T10:27:28","date_gmt":"2017-03-09T03:27:28","guid":{"rendered":"http:\/\/demo.prc.maiatech.com.vn\/?p=562"},"modified":"2017-07-13T00:05:13","modified_gmt":"2017-07-12T17:05:13","slug":"nghien-cuu-da-dang-di-truyen-lua-nep-dia-phuong","status":"publish","type":"post","link":"https:\/\/prc.org.vn\/?p=562","title":{"rendered":"Nghi\u00ean c\u1ee9u \u0111a d\u1ea1ng di truy\u1ec1n l\u00faa n\u1ebfp \u0111\u1ecba ph\u01b0\u01a1ng"},"content":{"rendered":"<table>\n<tbody>\n<tr>\n<td style=\"text-align: justify;\" colspan=\"2\">\u00a0\u00a0\u00a0 L\u00faa n\u1ebfp l\u00e0 m\u1ed9t trong nh\u1eefng nh\u00f3m l\u00faa \u0111\u1eb7c s\u1ea3n l\u00e2u \u0111\u1eddi c\u1ee7a nh\u00e2n d\u00e2n Vi\u1ec7t Nam v\u00e0 g\u1eafn li\u1ec1n v\u1edbi v\u0103n h\u00f3a truy\u1ec1n th\u1ed1ng v\u00e0 \u1ea9m th\u1ef1c Vi\u1ec7t Nam. L\u00faa n\u1ebfp c\u00f3 m\u1eb7t \u1edf h\u1ea7u h\u1ebft c\u00e1c t\u1ec9nh trong c\u1ea3 n\u01b0\u1edbc v\u00e0 \u0111\u01b0\u1ee3c tr\u1ed3ng \u1edf c\u1ea3 n\u01b0\u01a1ng v\u00e0 ru\u1ed9ng. Vi\u1ec7c nghi\u00ean c\u1ee9u \u0111a d\u1ea1ng di truy\u1ec1n t\u1eadp \u0111o\u00e0n l\u00faa n\u1ebfp kh\u00f4ng ch\u1ec9 c\u00f3 \u00fd ngh\u0129a trong vi\u1ec7c b\u1ea3o t\u1ed3n c\u00e1c gi\u1ed1ng l\u00faa \u0111\u1eb7c s\u1ea3n m\u00e0 c\u00f2n c\u00f3 \u00fd ngh\u0129a quan tr\u1ecdng trong c\u00f4ng t\u00e1c ch\u1ecdn t\u1ea1o gi\u1ed1ng l\u00faa ch\u1ea5t l\u01b0\u1ee3ng cao.<br \/>\nNg\u00e0y nay, ch\u1ec9 th\u1ecb ph\u00e2n t\u1eed \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng r\u1ed9ng r\u00e3i nh\u01b0 m\u1ed9t c\u00f4ng c\u1ee5 h\u1eefu hi\u1ec7u trong nghi\u00ean c\u1ee9u di truy\u1ec1n v\u00e0 cho ph\u00e9p \u0111\u00e1nh gi\u00e1 m\u1ed9t s\u1ed1 l\u01b0\u1ee3ng l\u1edbn locut tr\u1ea3i kh\u1eafp b\u1ed9 gen c\u1ee7a nhi\u1ec1u lo\u00e0i c\u00e2y tr\u1ed3ng. Nh\u1eefng th\u00f4ng tin v\u1ec1 \u0111a d\u1ea1ng di truy\u1ec1n \u1edf m\u1ee9c \u0111\u1ed9 ADN c\u00f3 th\u1ec3 ph\u00e1t hi\u1ec7n s\u1ef1 kh\u00e1c bi\u1ec7t nh\u1ecf nh\u1ea5t gi\u1eefa c\u00e1c gi\u1ed1ng, v\u00ec th\u1ebf gi\u00fap nh\u1eadn d\u1ea1ng nh\u1eefng gi\u1ed1ng qu\u00fd, hi\u1ebfm trong c\u00e1c t\u1eadp \u0111o\u00e0n c\u00e2y tr\u1ed3ng tr\u1ed3ng m\u1ed9t c\u00e1ch nhanh ch\u00f3ng v\u00e0 ch\u00ednh x\u00e1c. K\u1ef9 thu\u1eadt DNA fingerprinting \u0111\u00e3 \u0111\u01b0\u1ee3c \u00e1p d\u1ee5ng \u0111\u1ec3 nh\u1eadn d\u1ea1ng v\u00e0 ph\u00e2n bi\u1ec7t gi\u1ed1ng \u1edf h\u01a1n 30 lo\u1ea1i c\u00e2y tr\u1ed3ng kh\u00e1c nhau nh\u01b0 cam chanh (<em>Citrus spp.), <\/em>chu\u1ed1i<em> (Musa spp.), <\/em>l\u00faa m\u1ef3 (<em>Triticum<\/em> <em>spp.),<\/em> d\u00e2u t\u00e2y&#8230; (Bhat v\u00e0 ctv, 2006; Rinehart v\u00e0 ctv, 2007).<br \/>\nTrong s\u1ed1 c\u00e1c ch\u1ec9 th\u1ecb ADN th\u00ec ch\u1ec9 th\u1ecb SSR \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng r\u1ed9ng r\u00e3i v\u00e0 hi\u1ec7u qu\u1ea3 trong nghi\u00ean c\u1ee9u c\u1ea5u tr\u00fac di truy\u1ec1n l\u00faa tr\u1ed3ng <em>O. sativa<\/em>, nghi\u00ean c\u1ee9u qu\u00e1 tr\u00ecnh ti\u1ebfn h\u00f3a, l\u00e0m r\u00f5 \u0111\u1ed9 thu\u1ea7n c\u1ee7a v\u1eadt li\u1ec7u lai t\u1ea1o gi\u1ed1ng&#8230;, do \u0111\u00e2y l\u00e0 ch\u1ec9 th\u1ecb \u0111\u1ed3ng tr\u1ed9i cho \u0111a h\u00ecnh cao v\u00e0 \u1ed5n \u0111\u1ecbnh. Hi\u1ec7n nay, h\u01a1n 15.000 ch\u1ec9 th\u1ecb SSR \u0111\u00e3 \u0111\u01b0\u1ee3c thi\u1ebft l\u1eadp (<u>www.gramene.org<\/u>, 2006), ph\u1ee7 k\u00edn tr\u00ean b\u1ea3n \u0111\u1ed3 li\u00ean k\u1ebft di truy\u1ec1n c\u1ee7a l\u00faa (Giarrocco v\u00e0 ctv, 2007). Trong nh\u1eefng n\u0103m g\u1ea7n \u0111\u00e2y, nhi\u1ec1u c\u00f4ng tr\u00ecnh s\u1eed d\u1ee5ng ch\u1ec9 th\u1ecb SSR nghi\u00ean c\u1ee9u \u0111a d\u1ea1ng di truy\u1ec1n v\u00e0 DNA fingerprinting \u0111\u1ec3 nh\u1eadn d\u1ea1ng gi\u1ed1ng \u1edf l\u00faa \u0111\u00e3 \u0111\u01b0\u1ee3c c\u00f4ng b\u1ed1 (Kalyan v\u00e0 ctv, 2006; Jalaluddin v\u00e0 ctv, 2007; Muhammad v\u00e0 ctv, 2009; Navraj v\u00e0 ctv, 2009).<br \/>\nTrong nghi\u00ean c\u1ee9u n\u00e0y, ch\u1ec9 th\u1ecb SSR \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng \u0111\u1ec3 nghi\u00ean c\u1ee9u \u0111a d\u1ea1ng di truy\u1ec1n v\u00e0 l\u1eadp ti\u00eau b\u1ea3n ADN c\u1ee7a 45 ngu\u1ed3n gen l\u00faa n\u1ebfp \u1edf \u0111\u1ed3ng b\u1eb1ng mi\u1ec1n B\u1eafc, gi\u00fap nh\u1eadn d\u1ea1ng gi\u1ed1ng ph\u1ee5c v\u1ee5 c\u00f4ng t\u00e1c b\u1ea3o t\u1ed3n v\u00e0 cung c\u1ea5p th\u00f4ng tin c\u00f3 gi\u00e1 tr\u1ecb cho c\u00f4ng t\u00e1c ch\u1ecdn t\u1ea1o gi\u1ed1ng l\u00faa n\u1ebfp.<br \/>\nK\u1ebft qu\u1ea3 nghi\u00ean c\u1ee9u cho th\u1ea5y:<br \/>\n45 trong s\u1ed1 50 ch\u1ec9 th\u1ecb SSR cho \u0111a h\u00ecnh gi\u1eefa c\u00e1c gi\u1ed1ng l\u00faa n\u1ebfp t\u1ea1i 46 locut. T\u1ed5ng s\u1ed1 alen ph\u00e1t hi\u1ec7n t\u1ea1i 46 locut SSR c\u1ee7a 45 gi\u1ed1ng l\u00faa n\u1ebfp l\u00e0 234. S\u1ed1 alen \u0111a h\u00ecnh t\u1ea1i m\u1ed7i locut bi\u1ebfn \u0111\u1ed9ng t\u1eeb 2 \u0111\u1ebfn 10, \u0111\u1ea1t trung b\u00ecnh 5 alen\/locut. Trong s\u1ed1 45 ch\u1ec9 th\u1ecb SSR cho \u0111a h\u00ecnh, 18 ch\u1ec9 th\u1ecb ph\u00e1t hi\u1ec7n \u0111\u01b0\u1ee3c 28 alen duy nh\u1ea5t (alen hi\u1ebfm) \u1edf 16 gi\u1ed1ng. Nh\u1eefng th\u00f4ng tin thu \u0111\u01b0\u1ee3c t\u1eeb c\u00e1c ch\u1ec9 th\u1ecb n\u00e0y c\u00f3 th\u1ec3 c\u00f3 \u00edch cho c\u00f4ng t\u00e1c ph\u00e2n bi\u1ec7t v\u00e0 nh\u1eadn d\u1ea1ng 16 gi\u1ed1ng l\u00faa nghi\u00ean c\u1ee9u.<br \/>\n\u0110a s\u1ed1 c\u00e1c gi\u1ed1ng l\u00faa n\u1ebfp \u0111\u1ed3ng b\u1eb1ng (43\/45 gi\u1ed1ng) nghi\u00ean c\u1ee9u thu\u1ed9c nh\u00f3m l\u00faa <em>Japonica <\/em>d\u1ef1a tr\u00ean ph\u00e2n t\u00edch UPGMA t\u1eeb c\u00e1c ch\u1ec9 th\u1ecb gen nh\u00e2n SSR v\u00e0 ph\u00e2n t\u00edch ADN l\u1ee5c l\u1ea1p v\u1edbi c\u00e1c gi\u1ed1ng \u0111\u1ed1i ch\u1ee9ng Nipponbare v\u00e0 Kasalath<em>. <\/em>Hai gi\u1ed1ng l\u00faa n\u1ebfp c\u00f2n l\u1ea1i l\u00e0 N\u1ebfp n\u00f5n tre (6196) v\u00e0 N\u1ebfp h\u1ea1t chanh (7055) \u0111\u01b0\u1ee3c t\u00e1ch ri\u00eang th\u00e0nh m\u1ed9t nh\u00f3m v\u00e0 c\u00f3 ADN l\u1ee5c l\u1ea1p thu\u1ed9c nh\u00f3m l\u00faa <em>Indica.<\/em><br \/>\n\u1ee8ng d\u1ee5ng \u0111a h\u00ecnh ADN trong ph\u00e2n t\u00edch m\u1ed1i quan h\u1ec7 di truy\u1ec1n v\u00e0 ph\u00e2n lo\u1ea1i \u1edf ngu\u1ed3n gen l\u00faa n\u1ebfp trong c\u00f4ng tr\u00ecnh n\u00e0y c\u00f3 th\u1ec3 cung c\u1ea5p nh\u1eefng th\u00f4ng tin h\u1eefu \u00edch cho c\u00f4ng t\u00e1c nh\u1eadn d\u1ea1ng gi\u1ed1ng, gi\u00fap \u00edch cho c\u00f4ng t\u00e1c b\u1ea3o t\u1ed3n ngu\u1ed3n gen b\u1ea3n \u0111\u1ecba v\u00e0 ch\u01b0\u01a1ng tr\u00ecnh ch\u1ecdn gi\u1ed1ng l\u00faa n\u1ebfp \u1edf n\u01b0\u1edbc ta.<br \/>\nXem b\u00e1o c\u00e1o chi ti\u1ebft <a href=\"https:\/\/www.prc.maiatech.com.vn\/UserFiles\/File\/Bao%20cao%20khoa%20hoc\/Da%20dang%20di%20truyen%20lua%20nep%20dia%20phuong.pdf\">t\u1ea1i \u0111\u00e2y<\/a><br \/>\n<em>\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0<\/em><strong>\u00a0\u00a0<\/strong><\/td>\n<\/tr>\n<tr style=\"text-align: justify;\">\n<td style=\"text-align: right;\" colspan=\"2\">Tr\u1ea7n Danh S\u1eedu v\u00e0 CS<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n","protected":false},"excerpt":{"rendered":"<p>\u00a0\u00a0\u00a0 L\u00faa n\u1ebfp l\u00e0 m\u1ed9t trong nh\u1eefng nh\u00f3m l\u00faa \u0111\u1eb7c s\u1ea3n l\u00e2u \u0111\u1eddi c\u1ee7a nh\u00e2n d\u00e2n Vi\u1ec7t Nam v\u00e0 g\u1eafn<\/p>\n","protected":false},"author":4,"featured_media":1285,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[47],"tags":[],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/prc.org.vn\/index.php?rest_route=\/wp\/v2\/posts\/562"}],"collection":[{"href":"https:\/\/prc.org.vn\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/prc.org.vn\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/prc.org.vn\/index.php?rest_route=\/wp\/v2\/users\/4"}],"replies":[{"embeddable":true,"href":"https:\/\/prc.org.vn\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=562"}],"version-history":[{"count":2,"href":"https:\/\/prc.org.vn\/index.php?rest_route=\/wp\/v2\/posts\/562\/revisions"}],"predecessor-version":[{"id":1399,"href":"https:\/\/prc.org.vn\/index.php?rest_route=\/wp\/v2\/posts\/562\/revisions\/1399"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/prc.org.vn\/index.php?rest_route=\/wp\/v2\/media\/1285"}],"wp:attachment":[{"href":"https:\/\/prc.org.vn\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=562"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/prc.org.vn\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=562"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/prc.org.vn\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=562"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}