{"id":819,"date":"2017-04-05T09:57:24","date_gmt":"2017-04-05T02:57:24","guid":{"rendered":"http:\/\/demo.prc.maiatech.com.vn\/?p=819"},"modified":"2017-04-05T10:57:34","modified_gmt":"2017-04-05T03:57:34","slug":"nghien-cuu-quy-trinh-nhan-giong-in-vitro-cay-nua-konjac-amorphophallus-konjac","status":"publish","type":"post","link":"https:\/\/prc.org.vn\/?p=819","title":{"rendered":"Nghi\u00ean c\u1ee9u quy tr\u00ecnh nh\u00e2n gi\u1ed1ng in vitro c\u00e2y n\u01b0a Konjac (Amorphophallus konjac)"},"content":{"rendered":"\n\n\n\n
\u00a0\u00a0\u00a0\u00a0 N\u01b0a konjac l\u00e0 m\u1ed9t trong nh\u1eefng lo\u00e0i c\u00e2y tr\u1ed3ng \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng v\u00e0o c\u00f4ng ngh\u1ec7 th\u1ef1c ph\u1ea9m, y d\u01b0\u1ee3c, c\u00e2y c\u00f3 gi\u00e1 tr\u1ecb kinh t\u1ebf cao. Th\u00e0nh ph\u1ea7n ch\u00ednh trong c\u00e2y n\u01b0a \u0111\u01b0\u1ee3c g\u1ecdi l\u00e0 glucomannan – m\u1ed9t polysaccharide bao g\u1ed3m glucose v\u00e0 mannose v\u1edbi t\u1ef7 l\u1ec7 mol 2:3, \u0111\u01b0\u1ee3c n\u1ed1i v\u1edbi nhau b\u1edfi li\u00ean k\u1ebft \u03b2-1, 4. Nghi\u00ean c\u1ee9u l\u00e2m s\u00e0ng cho th\u1ea5y glucomannan c\u00f3 kh\u1ea3 n\u0103ng l\u00e0m gi\u1ea3m lipid, gi\u1ea3m huy\u1ebft \u00e1p t\u00e2m thu v\u00e0 gi\u1ea3m \u0111\u01b0\u1eddng huy\u1ebft.<\/p>\n

\u0110\u00e3 c\u00f3 m\u1ed9t v\u00e0i nghi\u00ean c\u1ee9u trong vi\u1ec7c t\u00e1i sinh m\u1ed9t s\u1ed1 lo\u00e0i trong chi N\u01b0a. Tuy nhi\u00ean k\u1ebft qu\u1ea3 nghi\u00ean c\u1ee9u c\u1ee7a ch\u00fang t\u00f4i cho th\u1ea5y m\u1eabu c\u1ea5y t\u1eeb c\u1ee7 l\u00e0 lo\u1ea1i m\u00f4 cho kh\u1ea3 n\u0103ng nh\u00e2n gi\u1ed1ng c\u00e2y n\u01b0a konjac th\u00f4ng qua m\u00f4 s\u1eb9o. Kh\u1ea3 n\u0103ng t\u00e1i sinh ch\u1ed3i c\u00f3 th\u1ec3 \u0111\u01b0\u1ee3c \u00e1p d\u1ee5ng \u0111\u1ec3 cung c\u1ea5p ngu\u1ed3n gi\u1ed1ng ch\u1ea5t l\u01b0\u1ee3ng nh\u1eb1m n\u00e2ng cao s\u1ea3n l\u01b0\u1ee3ng v\u00e0 h\u00e0m l\u01b0\u1ee3ng glucomannan trong c\u1ee7 ph\u1ee5c v\u1ee5 ng\u00e0nh c\u00f4ng nghi\u1ec7p th\u1ef1c ph\u1ea9m v\u00e0 y d\u01b0\u1ee3c.<\/p>\n

K\u1ebft qu\u1ea3 nghi\u00ean c\u1ee9u cho th\u1ea5y:<\/p>\n

– M\u00f4i tr\u01b0\u1eddng c\u00f3 2,4-D 1mg\/L, IBA 0,1 mg\/L\u00a0 v\u00e0 BA 3 mg\/L cho m\u00f4 s\u1eb9o c\u00f3 kh\u1ea3 n\u0103ng ph\u00e1t tri\u1ec3n ch\u1ed3i cao nh\u1ea5t (81,667%);<\/p>\n

– M\u00f4i tr\u01b0\u1eddng b\u1ed5 sung BA 3 mg\/L v\u00e0 IBA 0,2 mg\/L \u0111\u01b0\u1ee3c ch\u1ecdn l\u00e0 t\u1ed1i \u01b0u cho s\u1ef1 h\u00ecnh th\u00e0nh v\u00e0 ph\u00e1t tri\u1ec3n ch\u1ed3i n\u01b0a;<\/p>\n

– M\u00f4i tr\u01b0\u1eddng t\u00e1i sinh c\u00e2y t\u1ed1t nh\u1ea5t l\u00e0 m\u00f4i tr\u01b0\u1eddng MS c\u00f3 b\u1ed5 sung 0,1mg\/l IBA v\u1edbi s\u1ed1 r\u1ec5 trung b\u00ecnh \u0111\u1ea1t \u0111\u01b0\u1ee3c l\u00e0 9 r\u1ec5\/c\u00e2y, chi\u1ec1u d\u00e0i r\u1ec5 trung b\u00ecnh l\u00e0 1,16 cm v\u00e0 chi\u1ec1u cao c\u00e2y trung b\u00ecnh 12,33 cm;<\/p>\n

– Nh\u1eefng c\u00e2y con in vitro <\/em>c\u00f3 b\u1ed9 r\u1ec5 ph\u00e1t tri\u1ec3n ho\u00e0n ch\u1ec9nh \u0111\u01b0\u1ee3c r\u1eeda d\u01b0\u1edbi v\u00f2i n\u01b0\u1edbc ch\u1ea3y v\u00e0 tr\u1ed3ng trong nh\u1eefng ch\u1eadu nh\u1ef1a c\u00f3 ch\u1ee9a h\u1ed7n h\u1ee3p \u0111\u1ea5t v\u01b0\u1eddn:c\u00e1t:ph\u00e2n h\u1eefu c\u01a1 (1:1:1). Trong nh\u1eefng ng\u00e0y \u0111\u1ea7u m\u1edbi chuy\u1ec3n ra \u0111i\u1ec1u ki\u1ec7n ex vitro<\/em>, c\u00e2y con c\u1ea7n \u0111\u01b0\u1ee3c ph\u1ee7 l\u1ea1i b\u1eb1ng t\u00fai PE \u0111\u1ec3 duy tr\u00ec \u0111\u1ed9 \u1ea9m. Sau 4 tu\u1ea7n, nh\u1eefng c\u00e2y con n\u00e0y \u0111\u01b0\u1ee3c chuy\u1ec3n ra v\u01b0\u1eddn \u01b0\u01a1m v\u1edbi t\u1ec9 l\u1ec7 s\u1ed1ng s\u00f3t \u0111\u1ea1t tr\u00ean 90%.<\/p>\n

Xem b\u00e0i b\u00e1o chi ti\u1ebft t\u1ea1i \u0111\u00e2y<\/a><\/td>\n<\/tr>\n

B\u00f9i \u0110\u00ecnh Th\u1ea1ch, Nguy\u1ec5n Ph\u00f9ng H\u00e0 & cs<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n","protected":false},"excerpt":{"rendered":"

\u00a0\u00a0\u00a0\u00a0 N\u01b0a konjac l\u00e0 m\u1ed9t trong nh\u1eefng lo\u00e0i c\u00e2y tr\u1ed3ng \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng v\u00e0o c\u00f4ng ngh\u1ec7 th\u1ef1c ph\u1ea9m, y d\u01b0\u1ee3c,<\/p>\n","protected":false},"author":4,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[39],"tags":[],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/prc.org.vn\/index.php?rest_route=\/wp\/v2\/posts\/819"}],"collection":[{"href":"https:\/\/prc.org.vn\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/prc.org.vn\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/prc.org.vn\/index.php?rest_route=\/wp\/v2\/users\/4"}],"replies":[{"embeddable":true,"href":"https:\/\/prc.org.vn\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=819"}],"version-history":[{"count":1,"href":"https:\/\/prc.org.vn\/index.php?rest_route=\/wp\/v2\/posts\/819\/revisions"}],"predecessor-version":[{"id":820,"href":"https:\/\/prc.org.vn\/index.php?rest_route=\/wp\/v2\/posts\/819\/revisions\/820"}],"wp:attachment":[{"href":"https:\/\/prc.org.vn\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=819"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/prc.org.vn\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=819"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/prc.org.vn\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=819"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}