{"id":883,"date":"2017-04-08T14:48:09","date_gmt":"2017-04-08T07:48:09","guid":{"rendered":"http:\/\/demo.prc.maiatech.com.vn\/?p=883"},"modified":"2017-07-08T19:08:40","modified_gmt":"2017-07-08T12:08:40","slug":"xay-dung-tieu-ban-adn-cua-mot-so-giong-lua-dia-phuong-bang-chi-thi-snp-single-nucleotide-polymorphic","status":"publish","type":"post","link":"https:\/\/prc.org.vn\/?p=883","title":{"rendered":"X\u00e2y d\u1ef1ng ti\u00eau b\u1ea3n ADN c\u1ee7a m\u1ed9t s\u1ed1 gi\u1ed1ng l\u00faa \u0111\u1ecba ph\u01b0\u01a1ng b\u1eb1ng ch\u1ec9 th\u1ecb SNP (Single Nucleotide Polymorphic)"},"content":{"rendered":"<table>\n<tbody>\n<tr>\n<td colspan=\"2\">\u00a0\u00a0\u00a0 Nghi\u00ean c\u1ee9u \u0111\u00e3 ti\u1ebfn h\u00e0nh x\u00e2y d\u1ef1ng ti\u00eau b\u1ea3n ADN (DNA Fingerprinting) c\u1ee7a 26 ngu\u1ed3n gien l\u00faa \u0111\u1ecba ph\u01b0\u01a1ng s\u1eed d\u1ee5ng SNP chip bao g\u1ed3m 288 ch\u1ec9 th\u1ecb SNP l\u1ea5y t\u1eeb b\u1ed9 384-SNP GoldenGate (<em>Indica<\/em> x <em>Indica)<\/em> \u0111\u01b0\u1ee3c \u0111\u1ecdc b\u1eb1ng h\u1ec7 th\u1ed1ng Fluidigm EP1. K\u1ebft qu\u1ea3 ph\u00e2n t\u00edch cho th\u1ea5y 277 trong t\u1ed5ng s\u1ed1 288 ch\u1ec9 th\u1ecb cho c\u00e1c alen \u0111a h\u00ecnh, trung b\u00ecnh h\u1ec7 s\u1ed1 th\u00f4ng tin \u0111a h\u00ecnh (PIC) l\u00e0 0,27. Hai m\u01b0\u01a1i m\u1ed1t ch\u1ec9 th\u1ecb n\u1eb1m tr\u00ean 11 NST cho c\u00e1c alen hi\u1ebfm gi\u00fap ph\u00e2n bi\u1ec7t \u0111\u01b0\u1ee3c 9 ngu\u1ed3n gien l\u00faa. K\u1ebft qu\u1ea3 ph\u00e2n t\u00edch quan h\u1ec7 di truy\u1ec1n cho th\u1ea5y c\u00e1c gi\u1ed1ng l\u00faa nghi\u00ean c\u1ee9u c\u00f3 kho\u1ea3ng c\u00e1ch di truy\u1ec1n gi\u1eefa c\u00e1c ngu\u1ed3n gien \u0111\u1ea1t t\u1eeb 0,0379 \u0111\u1ebfn 0,5399 v\u00e0 chia l\u00e0m 3 nh\u00f3m ch\u00ednh. Tuy nghi\u00ean c\u1ee9u kh\u00f4ng thu \u0111\u01b0\u1ee3c hai ph\u00e2n nh\u00f3m <em>Indica<\/em> v\u00e0 <em>Japonica<\/em> ho\u00e0n to\u00e0n t\u00e1ch bi\u1ec7t, nh\u01b0ng c\u00e1c gi\u1ed1ng l\u00faa <em>Japonica <\/em>t\u1eadp trung th\u00e0nh m\u1ed9t nh\u00f3m v\u00e0 c\u00f3 quan h\u1ec7 di truy\u1ec1n g\u1ea7n g\u0169i h\u01a1n so v\u1edbi c\u00e1c gi\u1ed1ng l\u00faa <em>Indica<\/em>. Trong nh\u00f3m <em>Japonica<\/em>, c\u00e1c gi\u1ed1ng l\u00faa n\u1ebfp nh\u00f3m th\u00e0nh m\u1ed9t nh\u00f3m c\u00f3 quan h\u1ec7 di truy\u1ec1n g\u1ea7n g\u0169i. C\u00e2y ph\u00e2n lo\u1ea1i \u0111\u00e3 cho th\u1ea5y m\u1ed1i quan h\u1ec7 g\u1ea7n g\u0169i gi\u1eefa c\u00e1c gi\u1ed1ng l\u00faa c\u00f3 c\u00f9ng xu\u1ea5t x\u1ee9 \u0111\u1ecba l\u00fd (N\u1ebfp Ngoi v\u00e0 n\u1ebfp \u0110en, Kh\u1ea9u mang, S\u00e9ng C\u00f9 hay T\u00e1m ti\u00eau v\u00e0 T\u00e1m \u1ea5p B\u1eb9), nh\u01b0ng b\u1ed9 ti\u00eau b\u1ea3n \u0111\u00e3 cung c\u1ea5p s\u1ef1 kh\u00e1c bi\u1ec7t v\u1ec1 di truy\u1ec1n gi\u1eefa c\u00e1c ngu\u1ed3n gien n\u00e0y. V\u00ec v\u1eady, b\u1ed9 ch\u1ec9 th\u1ecb 278 SNP c\u00f3 th\u1ec3 s\u1eed d\u1ee5ng trong x\u00e2y d\u1ef1ng ti\u00eau b\u1ea3n ADN \u0111\u1ec3 nh\u1eadn d\u1ea1ng, x\u00e1c \u0111\u1ecbnh c\u00e1c gi\u1ed1ng l\u00faa \u0111\u1ecba ph\u01b0\u01a1ng c\u1ee7a Vi\u1ec7t Nam c\u0169ng nh\u01b0 \u1ee9ng d\u1ee5ng ch\u1ec9 th\u1ecb n\u00e0y trong c\u00f4ng t\u00e1c ch\u1ecdn t\u1ea1o gi\u1ed1ng.<\/p>\n<p>&nbsp;<\/td>\n<\/tr>\n<tr>\n<td style=\"text-align: right;\" colspan=\"2\">B\u00f9i Th\u1ecb Thu Giang, Nguy\u1ec5n Th\u1ecb Thanh Th\u1ee7y<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n","protected":false},"excerpt":{"rendered":"<p>\u00a0\u00a0\u00a0 Nghi\u00ean c\u1ee9u \u0111\u00e3 ti\u1ebfn h\u00e0nh x\u00e2y d\u1ef1ng ti\u00eau b\u1ea3n ADN (DNA Fingerprinting) c\u1ee7a 26 ngu\u1ed3n gien l\u00faa \u0111\u1ecba ph\u01b0\u01a1ng<\/p>\n","protected":false},"author":4,"featured_media":1171,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[39],"tags":[],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/prc.org.vn\/index.php?rest_route=\/wp\/v2\/posts\/883"}],"collection":[{"href":"https:\/\/prc.org.vn\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/prc.org.vn\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/prc.org.vn\/index.php?rest_route=\/wp\/v2\/users\/4"}],"replies":[{"embeddable":true,"href":"https:\/\/prc.org.vn\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=883"}],"version-history":[{"count":1,"href":"https:\/\/prc.org.vn\/index.php?rest_route=\/wp\/v2\/posts\/883\/revisions"}],"predecessor-version":[{"id":884,"href":"https:\/\/prc.org.vn\/index.php?rest_route=\/wp\/v2\/posts\/883\/revisions\/884"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/prc.org.vn\/index.php?rest_route=\/wp\/v2\/media\/1171"}],"wp:attachment":[{"href":"https:\/\/prc.org.vn\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=883"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/prc.org.vn\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=883"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/prc.org.vn\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=883"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}